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Original Essays | December 12, 2009

Alexander McCall Smith: IMG The Courage of Others

0 comments By Alexander McCall Smith

I have recently written a novel about life in England during the Second World War. I felt some concern before I tackled this theme — the War... Continue »
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    La's Orchestra Saves the World

    Alexander McCall Smith
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Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology

Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology Cover

Synopses & Reviews

Publisher Comments:

This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein structure by using modern-day laboratory techniques. Genetics, cell biology, and organic chemistry are prerequisites.
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Table of Contents

Examine Enzyme Activity of alpha-Amylase

1 Starch Plate Assay

2 Quantitative Enzyme Assay

3 Factors Affecting Enzyme Function

Examine alpha-Amylase Proteins

4 Analysis of Protein Structure Using RasMol

5 Analysis of alpha-Amylase Proteins

5A SDS-PAGE

5B Western Blotting

Examine DNA Structure

6 Analysis of DNA Structure Using RasMol

7 Isolation of Chromosomal DNA from Bacillus licheniformis

Find the alpha-Amylase Gene

8 PCR Amplification and Labeling of Probe DNA

9 Southern Hybridization

9A Restriction Enzyme Cleavage of Chromosomal DNA

9B Denaturation and Transfer of DNA to a Membrane

9C Southern Hybridization and Detection

Clone the alpha-Amylase Gene

10 Cloning the alpha-Amylase Gene

10A Cleavage of Chromosomal DNA

10B Cleavage of Plasmid DNA

10C Ligation of Chromosomal and Plasmid DNA

10D Transformation

10E Identification of alpha-Amylase Clones

Analyze alpha-Amylase Clones

11 Verification and Mapping of alpha-Amylase Clones

11A Verification of alpha-Amylase Clones Using PCR

11B Isolation of Plasmid DNA from alpha-Amylase Clones

11C Restriction Cleavage and Mapping of alpha-Amylase Plasmid DNA

11D Preservation of Recombinant Strains

11E Southern Analysis of alpha-Amylase Plasmid DNA

12 Enzyme Activity of alpha-Amylase Clones

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Product Details

ISBN:
9780072416640
Subtitle:
DNA to Protein -- A Laboratory Project in Molecular Biology
Publisher:
McGraw-Hill Science/Engineering/Math McGraw-Hill Science/Engineering/Math
Author:
Lyons, Eilene
Author:
Lyons, Eilene M.
Author:
Thiel, Teresa
Author:
Bissen, Shirley
Author:
Bissen, Shirley T.
Location:
Boston
Subject:
Molecular biology
Subject:
Biotechnology
Subject:
Molecular cloning
Subject:
Amylases.
Subject:
Biology projects.
Subject:
Life Sciences - Biochemistry
Subject:
alpha-Amylase,DNA,Enzyme,Protein,Starch Plate Assay,Affecting Enzyme Function,Quantitative Enzyme Assay,Enzyme Activity,Molecular Biology,Laboratory Project,Molecular Biology Biotechnology,Analyze alpha-Amylase Clones
Copyright:
2002
Edition Number:
1
Publication Date:
August 2001
Binding:
Spiral, comb or coil bound book
Grade Level:
College/higher education:
Language:
English
Illustrations:
Yes
Pages:
192
Dimensions:
10.78x8.46x.24 in. 1.08 lbs.
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