This volume of Methods in Enzymology and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before.

Key Features

* This volume and its companion Volumes 280, 281, and 282 provide

* A collation of the most recent and useful methods for the identfication, preparation, and quantification of vitamins and coenzymes

* Details on physical, chemical, and biological properties of vitamins and coenzymes

* Chemical and biological syntheses of vitamins, coenzymes, and their analogs

* Aspects of transport and metabolism of vitamins and coenzymes

This current set of volumes (279-282) in the series collects research on new techniques and methodologies attendant to assays, isolations, and characterizations of vitamins, coenzymes, and those systems responsible for their biosynthesis, transport, and metabolism. Coverage of many methods allows for flexibility in choosing methods. Contains sections on ascorbic acid, thiamin phosphates and analogs, lipoic acid and derivatives, pantothenic acid and coenzyme A, and biotin and derivatives.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."

--ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists."

--JOURNAL OF CHROMATOGRAPHY

LOGIA

"A series that has established itself as a definitive reference for biochemists."

--JOURNAL OF CHROMATOGRAPHY

nsulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."

--ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists."

--JOURNAL OF CHROMATOGRAPHY

HROMATOGRAPHY

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"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."

--AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."

--ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists."

--JOURNAL OF CHROMATOGRAPHY

This volume of "Methods in Enzymology" and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before. This volume and its companion Volumes 280, 281, and 282 provide: a collation of the most recent and useful methods for the identfication, preparation, and quantification of vitamins and coenzymes; details on physical, chemical, and biological properties of vitamins and coenzymes; chemical and biological syntheses of vitamins, coenzymes, and their analogs; and aspects of transport and metabolism of vitamins and coenzymes.

hetases.

D.L. Dyer and H.M. Said, Biotin Uptake in Cultured Cell Lines.

Y. Xu and D. Beckett, Biotinyl-5-Adenylate Synthesis Catalyzed by Escherichia coli Repressor of Biotin Biosynthesis.

J. Hymes, K. Fleischlauer, and B. Wolf, Biotinidase in Serum and Tissues.

K. Hayakawa, K. Yoshikawa, J. Oizumi, and K. Yamauchi, Determination of Biotinidase Activity with Biotinyl-6-aminoquinoline as Substrate.

E. Livaniou, S.E. Kakabakos, G.P. Evangelatos, S.A. Evangelatos, and D.S. Ithakissios, Determination of Serum Biotinidase Activity with Radioiodinated Biotinylamide Analogs.

F. Kohen, H. Bagci, G. Barnard, E. Bayer, B. Gayer, D. Schindler, E. Ainbinder, and M. Wilchek, Preparation and Properties of Anti-Biotin Antibodies.

H.B. White III, Competitive Binding Assays for Biotin-Binding Proteins.

Subject Index.

Author Index.