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Original Essays | November 5, 2009

John Buntin: IMG Notes from the (Bibliographic) Underground

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For more than 60 years, Los Angeles's origins, its underbelly, and (yes) its blondes have fueled the imagination of writers and directors from... Continue »
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Gus Protocols: Using the Gus Gene as a Reporter of Gene Expression

Synopses & Reviews

Publisher Comments:

The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay.

GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.

Key Features

* First book on the GUS reporter system

* Up-to-date reference lists following each of the fourteen chapters

* Comb-bound for convenient bench-top use

* Written by leading authorities including R.A. Jefferson, inventor of the GUS assay

* Illustrated with color

* Easy-to-follow procedures for

* Using the GUS system in both plants and animals

* GUS detection by fluorometric, spectrophotometric, and histochemical methods

* Fast, automated assays

Background information on

* The gb-glucuronidase reaction

* Substrates

* Instrumentation

* Construction of GUS fusion vectors

Review:

"This manual does an excellent job of demonstrating both the power and the versatility of the GUS system in plants... If you think the GUS system may be of use to your studies, this text provides a broad range of protocols, practical tips, and limitations to help use the system efficiently."

--ANALYTICAL BIOCHEMISTRY

"Gus Protocols is a laboratory manual that will be of great value to the researcher."

--TRENDS IN BIOTECHNOLOGY

Synopsis:

ckground information on

* The b-glucuronidase reaction

* Substrates

* Instrumentation

* Construction of GUS fusion vectors

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Table of Contents

(Section Headings): The GUS Reporter Gene System. The GUS Assay. Histochemical Detection of GUS. Applications of GUS to Plant Genetic Analysis. Applications of GUS to Animal Genetic Anaylsis. Appendices. Index.

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Product Details

ISBN:
9780122740107
Subtitle:
Using the GUS Gene as a Reporter of Gene Expression
Editor:
Gallagher, Sean R.
Author:
Gallagher, Sean
Publisher:
Academic Press
Location:
San Diego :
Subject:
Genetics
Subject:
Cytology
Subject:
Biotechnology
Subject:
Genetic engineering
Subject:
Gene expression
Subject:
Biochemical markers
Subject:
Genetic engineering -- Technique.
Subject:
Beta-glucuronidase genes.
Subject:
Life Sciences - Cytology
Edition Description:
Includes bibliographical references and index.
Series Volume:
no. 90-108, pts. 1-2
Publication Date:
19920310
Binding:
Paperback
Language:
English
Illustrations:
Yes
Pages:
221
Dimensions:
8.93x6.17x.57 in. .75 lbs.
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