This guide to growing human cancer in vitro begins with an essential chapter on characterization. Subsequent chapters describe the development of serum-free media and then deal with three research applications--cell differentiation and its control; the growth of cells in three dimensions in vitro and in vivo; and the prediction of drug and radiation resistance. The second section describes, in uniform format, the culture of specific tumor types, covering collection and handling, disaggregation, tumor cell selection, culture methods, subculture and cloning, and tissue and tumor cell identification.
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