Biotechnology revolutionized traditional plant breeding programs. This rapid change produced new discussions on techniques and opportunities for commerce, as well as a fear of the unknown. Plant Development and Biotechnology addresses the major issues of the field, with chapters on broad topics written by specialists. The book applies an informal style that addresses the major aspects of development and biotechnology with minimal references, without sacrificing information or accuracy. Divided into five primary parts, this volume explores how the field emerged from its early theoretical base to the technical discipline of today. It also covers progress being made with genetically engineered plants, providing a snapshot of the field's controversial present. Part III discusses methods for preparing media, creating solutions and dilutions, and accomplishing sterile culture work. It investigates common methods for visualizing and documenting studies, and quantifying responses of tissue culture in research. Part IV delivers the essential foundation of plant tissue culture, introducing the three types of commonly used culture regeneration systems. Part V integrates propagation techniques with other methodologies for the modification and manipulation of germplasm. Part VI concludes with special sections. Subjects include in vitro plant pathology, recent research into genetic and phenotypic variation, the mechanics of commercial plant production, and the importance of clean cultures and problems associated with maintaining in vitro cultures. The final chapter analyzes entrepreneurship in the field and outlines the do's and don'ts to consider when launching an enterprise.

Trigiano (plant biotechnology and pathology, University of Tennessee) and Gray (horticulture, University of Florida) employ an informal style to address major aspects of plant development and biotechnology. The book reviews how the field emerged from its early theoretical roots and provides a snapshot of the field's controversial present, describing current progress in genetically engineered plants. Methods are detailed for preparing media, creating solutions and dilutions, performing sterile culture work, and quantifying responses of tissue culture in research.
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This book begins by analyzing how crop science emerged and provides a snapshot of the field's controversial present by exploring progress being made with genetically engineered plants. It then discusses methods for preparing media, creating solutions and dilutions, and accomplishing sterile culture work. It also investigates common methods for visualizing and documenting studies and quantifying responses of tissue culture in research, and delivers the essential foundation of plant tissue culture, introducing the three types of commonly used culture regeneration systems. The final section integrates propagation techniques with other methodologies for the modification of germplasm.

Covering everything from the history of plant tissue and cell culture to propagation and development concepts to crop improvement techniques, this useful reference defines protocols needed in the daily operations of tissue culture research laboratories.