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Molecular Imaging: Fret Microscopy and Spectroscopy

Molecular Imaging: Fret Microscopy and Spectroscopy Cover


Synopses & Reviews

Publisher Comments:

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Table of Contents

Foreword by Robert M. Clegg

1. Proteins and the Flow of Information in Cellular Function, Robert H. Kretsinger

2. Basics of Fluorescence and FRET, Zygmunt Gryczynski, Ignacy Gryczynski and Joseph R. Lakowicz

3. An Introduction to Filters and Mirrors for FRET, C. Michael Stanley

4. FRET Imaging in the Wide-Field Microscope, Fred Schaufele, Ignacio Demarco, and Richard N. Day

5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes, Horst Wallrabe and Margarida Barroso

6. Multiphoton FRET Microscopy for Protein Localization in Tissue, James D. Mills, James R. Stone, David O. Okonkwo, Ammasi Periasamy and Gregory A. Helm

7. FRET Data Analysis: The Algorithm, Ye Chen, Masilamani Elangovan, and Ammasi Periasamy

8. Photobleaching FRET Microscopy, Anne Kenworthy

9. Single-Molecule FRET, Sungchul Hohng and Taekjip Ha

10. FRET Measurements Using Multispectral Imaging, Raad Nashmi, Scott E. Fraser, Henry A. Lester, and Mary E. Dickinson

11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers, Glen Redford and Robert M. Clegg

12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging, V. Krishnan Ramanujan, Jian-Hua Zhang, Victoria. E. Centonze, and Brian Herman

13. Time-Correlated Single Photon Counting FLIM-FRET Microscopy for Protein Localization, Ye Chen and Ammasi Periasamy

14. Bioluminescence Resonance Energy Transfer: Techniques and Potential, Mohammed Soutto, Yao Xu, and Carl H. Johnson

15. Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy, Keith M. Berland

16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy, Eleonora Keating, Claire M. Brown, and Nils O. Petersen

Product Details

FRET Microscopy and Spectroscopy
Periasamy, Ammasi
Periasamy, Ammasi
Day, Richard N.
Day, Periasamy, Ammasi, Richard
null, Ammasi
Day, Richard N.
Periasamy, Ammasi
null, Richard N.
Day, Richard N.
Oxford University Press
Fluorescence microscopy
Fluorescence spectroscopy
Medicine | Physiology
Molecular biology
Methods in Physiology Series
Publication Date:
Grade Level:
Professional and scholarly
43 color photos, 16 halftones, 49 line i
7.2 x 10.1 x 1 in 2.656 lb

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Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Chemistry » Spectroscopy

Molecular Imaging: Fret Microscopy and Spectroscopy
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Product details 336 pages Oxford University Press - English 9780195177206 Reviews:
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