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1 Burnside Chemistry- Spectroscopy

Molecular Imaging: Fret Microscopy and Spectroscopy

by

Molecular Imaging: Fret Microscopy and Spectroscopy Cover

 

Synopses & Reviews

Publisher Comments:

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Table of Contents

Foreword by Robert M. Clegg


1. Proteins and the Flow of Information in Cellular Function, Robert H. Kretsinger


2. Basics of Fluorescence and FRET, Zygmunt Gryczynski, Ignacy Gryczynski and Joseph R. Lakowicz


3. An Introduction to Filters and Mirrors for FRET, C. Michael Stanley


4. FRET Imaging in the Wide-Field Microscope, Fred Schaufele, Ignacio Demarco, and Richard N. Day


5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes, Horst Wallrabe and Margarida Barroso


6. Multiphoton FRET Microscopy for Protein Localization in Tissue, James D. Mills, James R. Stone, David O. Okonkwo, Ammasi Periasamy and Gregory A. Helm


7. FRET Data Analysis: The Algorithm, Ye Chen, Masilamani Elangovan, and Ammasi Periasamy


8. Photobleaching FRET Microscopy, Anne Kenworthy


9. Single-Molecule FRET, Sungchul Hohng and Taekjip Ha


10. FRET Measurements Using Multispectral Imaging, Raad Nashmi, Scott E. Fraser, Henry A. Lester, and Mary E. Dickinson


11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers, Glen Redford and Robert M. Clegg


12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging, V. Krishnan Ramanujan, Jian-Hua Zhang, Victoria. E. Centonze, and Brian Herman


13. Time-Correlated Single Photon Counting FLIM-FRET Microscopy for Protein Localization, Ye Chen and Ammasi Periasamy


14. Bioluminescence Resonance Energy Transfer: Techniques and Potential, Mohammed Soutto, Yao Xu, and Carl H. Johnson


15. Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy, Keith M. Berland


16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy, Eleonora Keating, Claire M. Brown, and Nils O. Petersen


Product Details

ISBN:
9780195177206
Subtitle:
FRET Microscopy and Spectroscopy
Editor:
Periasamy, Ammasi
Editor:
Periasamy, Ammasi
Editor:
Day, Richard N.
Author:
Periasamy, Ammasi
Author:
null, Richard N.
Author:
Day, Periasamy, Ammasi, Richard
Author:
null, Ammasi
Author:
Day, Richard N.
Editor:
Day, Richard N.
Publisher:
Oxford University Press
Subject:
General
Subject:
Physiology
Subject:
Fluorescence microscopy
Subject:
Fluorescence spectroscopy
Subject:
Medicine | Physiology
Subject:
Molecular biology
Copyright:
Series:
Methods in Physiology Series
Publication Date:
20051013
Binding:
Hardback
Grade Level:
Professional and scholarly
Language:
English
Illustrations:
43 color photos, 16 halftones, 49 line i
Pages:
336
Dimensions:
7.2 x 10.1 x 1 in 2.656 lb

Related Subjects

Arts and Entertainment » Music » General
History and Social Science » World History » General
Humanities » Philosophy » General
Languages » ESL » General
Religion » Comparative Religion » General
Religion » Eastern Religions » Philosophy General
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Chemistry » Spectroscopy

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