Now in its third edition, Laboratory Exercises in Microbiology contains a variety of interactive activities and experiments that teach readers the basic concepts of microbiology. It covers methods that allow the safe movement or transfer of microbial cells from one type of growth environment, classification and identification of microbes, microbial biochemistry, and medical, food, and environmental microbiology. The labs are introduced in a clear and concise manner, while maintaining a reader-friendly tone.

 

Preface

Introduction: Laboratory Operations and Safety: Hand Washing

Part I General Microscopy and Aseptic Technique

1. Laboratory Safety: Introduction to the Microscope
2. Transfer and Isolation Techniques
   1. Microbes in the Environment

Part II Microbial Morphology, Differential Stains

3. Cultural and Cellular Morphology
4. Bacterial Growth
5. Gram Stain
   1. Acid-Fast Stain
6. Endospore Stain
   1. Capsule Stain
   2. Hanging Drop Technique
7. Fungi
8. Viruses-Visualization and enumerations
9. Parasitology

Part III Microbial Control and Biochemistry

10. Microbial Sensitivity Testing: Ultraviolet Light Sensitivity
    1. Heat Sensitivity
    2. Chemical Sensitivity
    3. Anti-biotic Sensitivity: Kirby-Bauer Technique
11. Bacterial Biochemistry
12. Gas Requirements of Microorganisms
13. Specialized Media

Part IV Medical Microbiology

14. Genetics
15. Epidemiology
16. Speciman-Handling Protocols
17. Specific Laboratory Tests
18. Serology
19. Identification of Enteric Pathogens: Traditional Methods
20. Identification of Enteric Pathogens: Rapid Identification Methods
21. Identification of Bacterial Unknown: The Gram-Negative Unknown
22. Identification of a Bacterial Unknown: The Gram-Positive Cocci

Part V Food and Environmental Microbiology

23. Identification and Quantification of Microbial Numbers in a Water Sample
24. Identification of Microbes in Beef and Poultry and the Quantification of Microbial Numbers
25. Soil Microbiology
    1. Photographic Atlas
26. Microbial Ecology
27. Biofilms

Bibliography

Appendix 1

Appendix 2

Appendix 3

Credits

Index