Laboratory Exercises in Microbiology

Now in its third edition, Laboratory Exercises in Microbiology contains a variety of interactive activities and experiments that teach readers the basic concepts of microbiology. It covers methods that allow the safe movement or transfer of microbial cells from one type of growth environment, classification and identification of microbes, microbial biochemistry, and medical, food, and environmental microbiology. The labs are introduced in a clear and concise manner, while maintaining a reader-friendly tone.

Preface.

Introduction: Laboratory Operations and Safety: Hand Washing.

Part I General Microscopy and Aseptic Technique.

1. Laboratory Safety: Introduction to the Microscope.

2. Transfer and Isolation Techniques.

a. Microbes in the Environment

Part II Microbial Morphology, Differential Stains.

3. Cultural and Cellular Morphology.

4. Bacterial Growth.

5. Gram Stain.

a. Acid-Fast Stain.

6. Endospore Stain.

a. Capsule Stain.

b. Hanging Drop Technique.

7. Fungi.

8. Viruses-Visualization and enumerations.

9. Parasitology.

Part III Microbial Control and Biochemistry.

10. Microbial Sensitivity Testing: Ultraviolet Light Sensitivity.

a. Heat Sensitivity.

b. Chemical Sensitivity.

c. Anti-biotic Sensitivity: Kirby-Bauer Technique.

11. Bacterial Biochemistry.

12. Gas Requirements of Microorganisms.

13. Specialized Media.

Part IV Medical Microbiology.

14. Genetics.

15. Epidemiology.

16. Speciman-Handling Protocols.

17. Specific Laboratory Tests.

18. Serology.

19. Identification of Enteric Pathogens: Traditional Methods.

20. Identification of Enteric Pathogens: Rapid Identification Methods.

21. Identification of Bacterial Unknown: The Gram-Negative Unknown.

22. Identification of a Bacterial Unknown: The Gram-Positive Cocci.

Part V Food and Environmental Microbiology.

23. Identification and Quantification of Microbial Numbers in a Water Sample.

24. Identification of Microbes in Beef and Poultry and the Quantification of Microbial Numbers.

25.Soil Microbiology.

a. Photographic Atlas.

26. Microbial Ecology.

27. Biofilms.

Bibliography.

Appendix 1.

Appendix 2.

Appendix 3.

Credits.

Index.