Synopses & Reviews
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.
Book News Annotation:
Since the 1995 first edition, the technology of test-tube
transcription and translation--used to study protein synthesis and
function--has improved to the point that it can be adapted to
high-throughput screening formats, and can compete with in vivo
expression systems for preparative purposes. Contributors from a wide
range of biomedical disciplines describe new protocols and survey
specific applications.
Annotation ©2007 Book News, Inc., Portland, OR (booknews.com)
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