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Other titles in the Methods in Molecular Biology series:
Methods in Molecular Biology #2120: Plant Hormones: Methods and Protocolsby Sean Cutler
Synopses & Reviews
The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideas about hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology, this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and cutting edge, Plant Hormones: Methods and Protocols, Second Edition is an essential guide for all plant scientists who are facing the complex and challenging questions that will determine the future of this vital field.
Book News Annotation:
The past ten years have seen an explosion in our understanding of plant hormones. In this text, 28 international researchers examine the most current genetic, biochemical, analytical and chemical biological approaches for understanding and dissecting plant hormone action. Designed for plant scientists, the text covers genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Each chapter includes an introduction, a list of necessary methods, step-by-step methods, and a notes section on troubleshooting and avoiding pitfalls. Revisions from the first to second edition are not stated. Annotation ©2009 Book News, Inc., Portland, OR (booknews.com)
Rev. ed. of: Plant hormone protocols / edited by Gregory A. Tucker and Jeremy A. Roberts. c2000.
Given the rapid increase in our understanding of plant hormone biology, this second edition of a comprehensive review could not have come at a better time. In its chapters, expert researchers explore the latest approaches to understanding plant hormone action.
Table of Contents
Plant Hormones: Methods and Protocols, Second Edition S. Cutler and D. Bonetta, eds. Table of Contents Preface Contributors 1. Using reverse genetics to develop small knockout collections for specific biological questions. J. Northey and P. McCourt 2. Visualizing auxin transport routes in Arabidopsis leaf primordia. D. Marcos and T. Berleth 3. Bioinformatic analysis of hormone microarray data. G.W. Bassel and N.J. Provart 4. Measurement of plant hormones by liquid chromatography-mass spectrometry. S.J. Owen and S.R. Abrams 5. Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry. M. Okamoto, A Hanada, Y Kamiya, S Yamaguchi and E Nambara 6. Pull-down assays for plant hormone research. S. Kepinski 7. Binding assays for brassinosteroid receptors. A. Caño-Delgado and Z-Y Wang 8. Binding assays for abscisic acid receptors. F.A. Razem and R.D. Hill 9. Cytokinin sensing systems using microorganisms. M. Higuchi, T. Kakimoto and T. Mizuno 10. Hormone-binding assay using living bacteria expressing eukaryotic receptors. G.A. Romanov and S.N. Lomin 11. Discovery of plant microRNAs and short-interfering RNAs by deep parallel sequencing. P. Chellappan and H. Jin 12. The use of chemical genomics to investigate pathways intersecting auxin-dependent responses and endomembrane trafficking in Arabidopsis thaliana L. Norambuena, G.R. Hicks and N.V. Raikhel
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