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Methods in Molecular Biology #601: Heterologous Expression of Membrane Proteins: Methods and Protocols

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Methods in Molecular Biology #601: Heterologous Expression of Membrane Proteins: Methods and Protocols Cover

 

Synopses & Reviews

Publisher Comments:

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. 

Book News Annotation:

This guide for new and experienced researchers details traditional and emerging methods and protocols for heterologous expression of membrane proteins. To begin, the book overviews different heterologous expression systems that produce membrane proteins for structural analysis. Methods and protocols are then described for each heterologous expression system proposed. Protocols contain lists of materials and reagents, step-by-step instructions, and notes on troubleshooting, accompanied by b&w illustrations and images. Wide margins offer space for readers' notes. Mus-Veteau is affiliated with the Institute of Developmental Biology and Cancer, France. Annotation ©2010 Book News, Inc., Portland, OR (booknews.com)

Synopsis:

This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution.

Table of Contents

Heterologous Expression of Membrane Proteins: Methods and Protocols Isabelle Mus-Veteau, Editor   Table of Contents Preface Contributors 1. Heterologous expression of membrane proteins for structural analysis Isabelle Mus-Veteau 2. Production of membrane proteins in E. coli and L. lactis Bert Poolman and Eric R. Geertsma 3. Mammalian membrane receptors expression as inclusion bodies in E. coli Bernard Mouillac and Jean-Louis Banères 4. Expression of membrane proteins at the E. coli membrane for structural studies Manuela Zoonens and Bruno Miroux 5. Membrane protein expression in Lactococcus lactis Annie Frelet-Barrand, Sylvain Boutigny, Edmund R. S. Kunji and Norbert Rolland 6. Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae Olivier Joubert, Rony Nehme, Michel Bidet and Isabelle Mus-Veteau 7. Mammalian membrane proteins expression in baculovirus infected insect cells Céline Trometer and Pierre Falson 8. Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G Protein-Coupled Receptor as a model Karl Brillet, Carlos A. Pereira, and Renaud Wagner 9. Membrane protein expression in the eyes of transgenic flies. Valérie Panneels and Irmgard Sinning 10. Expression of Mammalian Membrane Proteins in Mammalian Cells using Semliki Forest Virus Vectors Kenneth Lundstrom 11. Membrane Protein Expression in Cell-Free Systems Birgit Schneider, Friederike Junge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dötsch, Frank Bernhard 12. Practical considerations of membrane protein instability for purification and crystallisation Christopher G. Tate 13. Membrane protein solubilization Katia Duquesne and James N. Sturgis 14. Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro Cécile Breyton, Bernard Pucci and Jean-Luc Popot 15. Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPase Delphine Cardi, Cédric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc le Maire and Christine Jaxel

Product Details

ISBN:
9781607613435
Author:
Mus-veteau, Isabelle
Publisher:
Humana Press
Editor:
Mus-Veteau, Isabelle
Author:
Mus-Veteau, Isabelle
Subject:
Life Sciences - Cytology
Subject:
Life Sciences - Biochemistry
Subject:
Life Sciences - Biology - Molecular Biology
Subject:
Cytology
Subject:
Crystallization
Subject:
Heterologous expression systems
Subject:
Protein solubilization
Subject:
Stabilization
Subject:
Cell biology
Subject:
Proteomics
Subject:
Membrane Biology
Subject:
Biology-Cytology and Cell Biology
Subject:
Molecular biology
Copyright:
Edition Description:
2010
Series:
Methods in Molecular Biology
Series Volume:
601
Publication Date:
20091218
Binding:
HARDCOVER
Language:
English
Pages:
284
Dimensions:
10.10x7.20x1.00 in. 1.50 lbs.

Related Subjects


Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » General
Science and Mathematics » Biology » Molecular
Science and Mathematics » Biology » The Attic
Science and Mathematics » Chemistry » Biochemistry

Methods in Molecular Biology #601: Heterologous Expression of Membrane Proteins: Methods and Protocols New Hardcover
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Product details 284 pages Humana Press - English 9781607613435 Reviews:
"Synopsis" by , This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution.
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