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Enzyme Assays: A Practical Approach (Practical Approach Series)

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Enzyme Assays: A Practical Approach (Practical Approach Series) Cover

 

Synopses & Reviews

Publisher Comments:

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Book News Annotation:

Eisenthal and Danson (both of the Department of Biology and Biochemistry, U. of Bath, UK) explain commonly used and newer, experimental techniques of measuring the rate of enzyme-catalyst reaction as a way to begin to define the functional roles of genetic proteins. After an introductory chapter dealing with fundamental principles, chapters look at photometric, radiometric, high performance liquid chromatographic, and electrochemical techniques. Further topics include techniques for enzyme extraction, determination of active site concentration, high throughput screening, and statistical analysis of enzyme kinetic data.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Table of Contents

1. Principles of enzyme assay and kinetic studies, Keith F Tipton

2. Photometric assays, Robert A John

3. Radiometric assays, Kelvin T Hughes

4. High performance liquid chromatographic assays, Shabih E H Syed

5. Electrochemical assays: the oxygen electrode, J B Clark

6. Electrochemical assays: the nitric oxide electrode, R D Hurst and J B Clark

7. Electrochemical assays: the pH-stat, Keith Brocklehurst

8. Enzyme assays after gel electrophoresis, Gunter M Rothe

9. Techniques for enzyme extraction, Nicholas C Price and Lewis Stevens

10. Determination of active site concentration, Mark T Martin

11. High throughput screening - considerations for enzyme assays, David Hayes and Geoff Mellor

12. Statistical analysis of enzyme kinetic data, Athel Cornish-Bowden

Product Details

ISBN:
9780199638208
Editor:
Eisenthal, Robert
Editor:
Danson, Michael J.
Editor:
Eisenthal, Robert
Editor:
Danson, Michael J.
Author:
Michael Danson
Author:
Eisenthal, Robert
Author:
null, Michael
Author:
null, Robert
Publisher:
Oxford University Press, USA
Subject:
Biochemistry
Subject:
Enzymology
Subject:
Life Sciences - Biochemistry
Subject:
Life Sciences - Cytology
Subject:
Life Sciences | Biochemistry | Proteins
Subject:
Life Sciences | Biochemistry | Proteins and Enzymology
Subject:
Life Sciences | Biochemistry | Proteins & Enzymology
Subject:
Chemistry-Biochemistry
Edition Number:
2
Edition Description:
Paperback
Series:
Practical Approach Series
Series Volume:
90
Publication Date:
20020631
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
70 line, 2 halftone, 1 color plate
Pages:
302
Dimensions:
9.64x7.42x.65 in. 1.45 lbs.

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Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Chemistry » Biochemistry

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