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Original Essays | April 11, 2014

Paul Laudiero: IMG Shit Rough Draft

I was sitting in a British and Irish romantic drama class my last semester in college when the idea for Shit Rough Drafts hit me. I was working... Continue »
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Developmental Biology Protocols Volume 3


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Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.

Table of Contents

Part I. Introduction. Developmental Biology Protocols: Overview III, Tuan and Lo. Part II. Manipulation of Developmental Gene Expression and Function. Ectopic Expression in Drosophila, Wilder. Clonal Analysis in the Examination of Gene Function in Drosophila, Rooke, Theodosiou, and Xu. Application of Antisense Oligonucleotides in Developing Chicken Embryos, Alexander, Barnes, and Tuan. Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development, Oberlender and Tuan. Part III. Analysis of Gene Expression. Gene Expression Analyzed by Ribonuclease Protection Assay, Bennett. Relative RT-PCR, Giambernardi and Klebe. Gene Expression Analysis Using Quantitative RT-PCR and a Multispecific Internal Control, Shire and Legoux. In Situ PCR Detection of HIV Expression in the Human Placenta, Sheikh, Polliotti, and Miller. Gene Expression Analysis by In Situ Hybridization: Radioactive Probes, Wawersik and Epstein. Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification, Moorman, De Boer, Hagoort, Franco, and Lamers. mRNA and Protein Colocalization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry, Tuan. Whole-Mount In Situ Hybridization to Study Gene Expression during Mouse Development, Lowe and Kuehn. Multicolor Whole-Mount In Situ Hybridization, Hauptmann and Gerster. Methods for Double Detection of Gene Expression: Combined In Situ Hybridization and Immunohistochemistry or Histochemistry, Conlon. Visualization of the Expression of Green Fluorescent (GFP)-Linked Proteins, Ayoob, Jean Sanger, and Joseph Sanger. Part IV. Models of Morphogenesis and Development. Monoclonal Antibodies in the Analysis of Embryonic Development, Bedian. Mesoderm Induction in Xenopus: Oocyte Expression System and Animal Cap Assay, Yao, Kessler. Amphibian Organizer Activity, Niehrs. Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery, Packard, Cox, and Poole. Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration, Newgreen and Murphy. The Chimeric Human/Mouse Model of Angiogenesis, Petitclerc, von Schalscha, and Brooks. Analysis of Embryonic Vascular Morphogenesis, Sato and Bartunkova. Epithelial-Mesenchyme Interactions, Hall. Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning, Ros, Simandl, Clark, and Fallon. Palate Development--In Vitro Procedures, Pisano and Greene. Part V. In Vitro Models and Analysis Differentiation and Development. In Vitro Fertilization Heyner, Tucker. Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development, Peters, Chapman, and Soares. Bone Marrow-Derived Mesenchymal Progenitor Cells, Johnstone and Yoo. Identification, Characterization, and Differentiation of Human Prostate Cells, Mehta, Perez-Stable, Roos, and Nadji. Preparation of Chick Striated Muscle Cultures, DiLullo, George-Weinstein, and Gerhart. Study of Skeletal Myogenesis in Cultures of Unsegmented Somitic Mesoderm, Borycki and Emerson. Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Chondrocyte Maturation, DeLise, Stringa, Woodward, Mello, and Tuan. Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells, DeLise and Tuan. Murine Cells, Haas and Tuan. Skeletogenesis: In Vitro Analysis of Bone Cell Differentiation, Majolagbe and Robey. Studying Early Hematopoiesis Using Avian Blastoderm Cultures, Eisenberg. Isolation and Culture of Mouse Germ Cells, De Miguel and Donovan. Cadherin-Mediated Cell-Cell Interactions, Knudsen and Soler. Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Progeins, Underhill and Zhang. Microinjection of Fluorescently Labeled Alpha-Åctinin into Living Cells, Jean Sanger, Danowski, and Joseph Sanger. Pax-3 and Vertebrate Development, Epstein. Genetic-Engineered Models of Skeletal Diseases I. Collagen Type X, Jacenko. Genetic-Engineered Models of Skeletal Diseases II. Targeting Mutations into Transgenic Mice Chondrocytes, Garofalo and Horton. Transgenic Mouse Models of Craniofacial Disorders, Liu, Snead, and Maxson.

Product Details

Tuan, Rocky S.
Lo, Cecilia W.
Lo, Cecilia W.
Tuan, Rocky S.
Tuan, Rocky Ed.
Lo, Cecilia W.
Tuan, Rocky S.
Dat., Tuan
Humana Press
Developmental Biology
Molecular biology
Cancer cells
Killer cells
Life Sciences - Cytology
Life Sciences - Biology - Developmental Biology
Life Sciences - Biology - Molecular Biology
Edition Description:
Methods in Molecular Biology Paperback
Series Volume:
Publication Date:
254 x 178 mm 1164 gr

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Related Subjects

Health and Self-Help » Health and Medicine » General
Health and Self-Help » Health and Medicine » General Medicine
Science and Mathematics » Biology » General
Science and Mathematics » Biology » Molecular

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