Synopses & Reviews
Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.
Book News Annotation:
The advance in transgenic technologies to express cloned genes in the
mouse have led to the mouse becoming one of the most used animal
models for biomedical research. Hofker (Maastricht U., The
Netherlands) and van Deursen (Mayo Clinic, US) present 18 papers that
explain the reasons for picking particular protocols and then
describe the protocols in a step-by-step manner. In addition, two
chapters describe alternative strategies (random mutagenesis and
viral gene transduction) for studying gene function in the mouse.
Other topics include generating conditional mouse mutants via
tetracycline- controlled gene expression, manipulating large insert
clones for transgenesis, reporter molecules in genetically engineered
mice, and aggregation of embryos and embryonic stem cells.
Annotation c. Book News, Inc., Portland, OR (booknews.com)
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