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Doubled Haploid Production in Crop Plants: A Manual

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Doubled Haploid Production in Crop Plants: A Manual Cover

 

Synopses & Reviews

Publisher Comments:

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision. The user will also find information on organization of a doubled haploid laboratory, basic DH media and on associated simple cytogenetic methods for ploidy level analysis. The practical protocols are supplemented with the list of published DH protocols for other crop plants and separate chapters dealing with major applications of DH in breeding, mutant production, transgenesis, and genetic mapping and genomics.

The book was prepared by the Plant Breeding and Genetics Section of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture in close co-operation with the EU program `COST 851' on `Gametic cells and molecular breeding for crop improvement'.

Synopsis:

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision.

Product Details

ISBN:
9789048163939
Subtitle:
A Manual
Author:
Maluszynski, M.
Author:
Szarejko, I.
Author:
Kasha, K. J.
Author:
Forster, B. P.
Publisher:
Springer
Location:
Dordrecht
Subject:
Botany
Subject:
Plant Sciences
Subject:
Tree Biology
Subject:
Life Sciences - General
Subject:
Botany-General
Subject:
Life sciences
Subject:
Language, literature and biography
Subject:
Biomedical and Life Sciences
Subject:
Trees
Copyright:
Edition Description:
1st Edition. Softcover version of original hardcover edition 2003
Publication Date:
20110729
Binding:
Hardback
Language:
English
Pages:
474
Dimensions:
240 x 160 mm

Related Subjects

Engineering » Environmental Engineering » Forestry
Science and Mathematics » Biology » General
Science and Mathematics » Botany » General
Science and Mathematics » Botany » Trees and Shrubs
Science and Mathematics » Nature Studies » Trees

Doubled Haploid Production in Crop Plants: A Manual New Trade Paper
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Product details 474 pages Springer - English 9789048163939 Reviews:
"Synopsis" by , This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision.
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