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The Portrait of a Lady (World's Classics)
Synopses & Reviews
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the
laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes.
Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a
versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology.
This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and
reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.
When Isabel Archer, a young American woman with looks, wit, and imagination, arrives in Europe, she sees the world as 'a place of brightness, of free expression, of irresistible action'. She turns aside from suitors who offer her their wealth and devotion to follow her own path. But that way leads to disillusionment and a future as constricted as 'a dark narrow alley with a dead wall at the end'. In a conclusion that is one of the most moving in modern fiction, Isabel makes her final choice.
When Isabel Archer, a young American with looks, wit, and imagination, arrives in Europe, she sees the world as "a place of brightness," full of possibility. Rejecting suitors who offer her wealth and devotion, she follows her own path and finds it leads to a dark and constricted future. The Portrait of a Lady is the masterpiece of James's middle period, and Isabel is his most engaging central character. This edition provides a new introduction and notes, and includes Henry James's own Preface.
About the Author
Nicola Bradbury is Lecturer in English at Reading University.
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