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Methods in Cell Biology #96: Microtubules, in Vitro


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Publisher Comments:

There continues to be intense interest in the microtubule cytoskeleton, the assembly, structure and regulation of microtubules and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complimented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoublues in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cyctoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

* Chapters reflect both experimental procedures and new developments in the field of Microtubule in vitro research.

* Combines classical approaches and modern technologies

* Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

Table of Contents

Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes.

  1.  Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography
  2. Herbert P. Miller and Leslie Wilson

  3. Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity
  4. Adrian Begaye and Dan L Sackett

  5. Isolating Tubulin From Nonneural Sources
  6. Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette

  7. Characterization of Anti-b -Tubulin Antibodies
  8. Anthony J. Spano* and Anthony Frankfurter

  9. Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction
  10. Sharon Lobert, Laree Hiser and John J. Correia

  11. Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes
  12. Mónica López-Fanarraga, Gerardo Carranza, Raquel Castaño, Sofia Nolasco, J. Avila and J.C. Zabala

  13. Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins
  14. Redeker, Virginie

  15. Methods in Tubulin Proteomics
  16. Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard

    Section 2: Microtubule Structure and Dynamics

  17.  Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions
  18. Eva Nogales, Vincent H. Ramey and Hong-Wei Wang

  19. High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy
  20. Loic Hamon, Patrick A. Curmi, David Pastré

  21. Holly V. Goodson and Ivan V. Gregoretti
  22. Using computational modeling to understand microtubule dynamics: a primer for cell biologists

  23. Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev
  24. Mythili Yenjerla, Manu Lopus and Leslie Wilson

  25. Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers
  26. Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt

  27. Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy
  28. Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schäffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard

  29. Studying kinesin motors by optical 3D-nanometry in gliding motility assays
  30. Bert Nitzsche, Volker Bormuth, Corina Bräuer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez

    Section 3: Drugs

  31. Analysis of tubulin oligomers by Analytical Ultracentrifugation.
  32. J.J. Correia

  33.  Determination of Drug Binding to Microtubules in vitro
  34. Jennifer A. Smith and Mary Ann Jordan

  35. Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions
  36. Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda

  37. A Tubulin polymerization micro-assay used to compare ligand efficacy
  38. Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton.

  39. Fluorescent Taxoid Probes for Microtubule Research
  40. Isabel Barasoain, J. Fernando Díaz, and José M. Andreu

  41.  The binding of vinca domain agents to tubulin: structural and biochemical studies
  42. Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoît Gigant

    Section 4: Interactions with Motors and MAPs

  43. Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance
  44. Marie-Jeanne Clément, Philippe Savarin, Elisabeth Adjadj, André Sobel, Flavio Toma, Patrick A. Curmi

  45. Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR
  46. François Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot

  47. Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro - Focus on Tau
  48. Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein

  49. Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM)
  50. Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman

  51. Multiple color single molecule TIRF imaging and tracking of MAPs and motors
  52. Jennifer L. Ross and Ram Dixit

  53. Dixit and Ross - final submitted 11/4 & lost - resubmitted 1/8/10
  54. Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics
  55. Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey

    Section 5: Functional Extracts and Force Measurements

  56. Quantitative Characterization of filament dynamics by single-molecule lifetime measurements.
  57. Leonid A. Mirny and Daniel J. Needleman

  58. Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle.
  59. Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin

  60. In vitro assays to study force generation at dynamic microtubule ends
  61. Liedewij Laan and Marileen Dogterom

  62. Reconstitution and functional analysis of kinetochore subcomplexes
  63. Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman

  64. In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces

Ekaterina L. Grishchuk and Fazly I. Ataullakhanov

Product Details

Wilson, Leslie
Academic Press
Correia, John J.
Life Sciences - Biology - General
Life Sciences - Cytology
Research -- Methodology.
Biology-Cytology and Cell Biology
Edition Description:
Methods in Cell Biology
Series Volume:
Publication Date:
9.25 x 7.5 in

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Reference » Science Reference » General
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » General

Methods in Cell Biology #96: Microtubules, in Vitro New Hardcover
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