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Colloidal Gold: Principles, Method & Application

Colloidal Gold: Principles, Method & Application Cover


Synopses & Reviews

Publisher Comments:

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.

This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.

Key Features

* Among the first books to cover principles and methodology of colloidal gold in microscopy

* Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book

* Discusses problems and limitationsof techniques

* Guides users to avoid problems and choose the correct procedures for specific applications

* Contributors are eminent authorities in their fields

Topics covered in Volume 1 include:

* Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry

* Methods for preparing colloidal gold particles of different sizes

* Protein A-gold, protein G-gold, and lectin-gold techniques

* The use of resins and thin cryosections

* Multiple labeling

Topics covered in Volume 2 include:

* Methodology for the study of antigenicity in neurology, virology, exocytosis, cytoskeleton, and plant tissues.

* The use of colloidal gold in conjunction with negative staining, nucleic acid hybridization, and darkfield and epipolarization microscopy

* The detection of proteins with colloidal gold

Molecular interactions between gold and proteins

* The roles of tissue processing in colloidal gold methods

* Low temperature methods

Topics covered in Volume 3 include:

* Production of monoclonal and polyclonal antibodies

* Lowicryl, polar, and apolar resins

* Preserving and localizing antigenicity; sectioning; high-resolution morphological localization

* Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation

* Applications to biomedical studies

* Colloidal gold in conjunction with video-enhanced microscopy

Table of Contents

Volume 1:
List of Contributors.
Contents of Other Volumes.
The Development and Application of Colloidal Gold as a Microscopic Probe, D.A. Handley.
Methods for Synthesis of Colloidal Gold, D.A. Handley.
Protein A-Gold and Protein G-Gold Postembedding Immunoelectron Microscopy, M. Bendayan.
Preparation and Application of Lectin-Gold Complexes, N. Benhamou.
Colloidal Gold for Multiple Staining, J. Doerr-Schott.
Immunogold Labeling of Ultrathin Cryosections, P.M.P. van Bergen en Henegouwen.
Colloidal Gold for Scanning Electron Microscopy, M. Horisberger.
Backscattered Electron Imaging of the Colloidal Gold Marker on Cell Surfaces, E. De Harven and D. Soligo.
Silver-Enhanced Colloidal Gold Method, L. Scopsi.
Silver-Enhanced Colloidal Gold for Light Microscopy, G.W. Hacker.
Strategies in Colloidal Gold Labeling of Cell Surfaces and Cytoskeletal Elements of Cultured Cells, G.B. Birrell and O. Hayes Griffith.
Colloidal Gold-Immunoreplica Method, <>M.V. Nermut and A. Nicol
Immunogold Double-Diffusion Method, J.R. Harris.
Colloidal Gold-Labeled Agarose-Gelatin Microspherule MethodK.-X. Gao.
Colloidal Gold for Microbiological Immunocytochemistry, J.E. Beesley.
Immunogold Labeling of Viruses in Suspension, E. Kjeldsberg.
Plasma Membrane Localization of Proteins with Gold Immunocytochemistry, A.N. van den Pol, M. Ellisman, and T. Deerinck.
Factors Affecting the Staining with Colloidal Gold, K. Park, H. Park, and R.M. Albrecht.
Each chapter includes references.

Volume 2:
List of Contributors.
Contents of Other Volumes.
Molecular Interactions between Colloidal Gold, Proteins, and Living Cells, P. Baudhuin, P. Van der Smissen, S. Beauvois, and P.J. Courtoy.
Protein A-Gold: Nonspecific Binding and Cross-Contamination, A.D. Hyatt.
Role of Tissue Processing in Colloidal Gold Methods, G.R. Newman and J.A. Hobot.
LR White Embedding Medium for Colloidal Gold Methods, G.R. Newman.
Lowicryls and Low-Temperature Embedding for Colloidal Gold Methods, J.A. Hobot.
The Enzyme-Gold Cytochemical Approach: A Review, M. Bendayan.
Preparation and Application of Lipoprotein-Gold Complex, D.A. Handley and C.M. Arbeeny.
Preparation and Application of Albumin-Gold Complex, IS. Villaschi
Label-Fracture Cytochemistry, F.W.K. Kan and P.P. da Silva.
Colloidal Gold Conjugates for Retrograde Neuronal Tracing, D. Menetrey and A.I. Basbaum.
Colloidal Gold Labeling of Microtubules in Cleaved Whole Mounts of Cells, J.A. Traas.
Colloidal Gold: Immunonegative Staining Method, J.E. Beesley.
Immunogold Labeling of Viruses in Situ, S.M. Pietschmann, E.H.S. Hausmann and H.R. Gelderblom.
Study of Exocytosis with Colloidal Gold and Other Methods, P. Buma.
Colloidal Gold Labeling of Acrylic Resin-Embedded Plant Tissues, E.M. Herman.
Preembedding Immunogold Staining of Cell Surface-Associated Antigens Performed on Suspended Cells and Tissue Sections, C. Ferrari, G. De Panfilis, and G.C. Manara.
Colloidal Gold in High-Voltage Electron Microscopy-Ruthenium Red Method and Whole-Cell Mount, K. Takata and H. Hirano.
Correlative Light and Electron Microscopic Immunocytochemistry in Reembedded Resin Sections with Colloidal Gold, H. Mar and T.N. Wight.
Streptavidin-Gold Labeling for Ultrastructural in Situ Nucleic Acid Hybridization, R.A. Wolber and T.F. Beals.
Detection of Proteins with Colloidal Gold, R. Rohringer.
Undecagold-Antibody Method, J.F. Hainfeld.
Immunogold Labeling for the Single-Laser FACS Analysis of Triple Antibody-Binding Cells, T.H. Totterman and R. Festin.
Silver-Enhanced Colloidal Gold for the Detection of Leukocyte Cell Surface Antigens in Dark-Field and Epipolarization Microscopy, M. De Waele.
Each chapter includes references.

Volume 3:
Contents of Other Volumes.
Some Basic Concepts for the Choice of Methods, E. Kellenberger and M.A. Hayat.
Techniques for the Production of Monoclonal and Polyclonal Antibodies, K.J. Morrow, Jr., E. Unuvar, S.W. King, and J.B. Mleczko.
Lowicryl Resins, W. Villiger.
Polar or Apolar Lowicryl Resin for Immunolabeling?, M. Durrenberger, W. Villiger, B. Arnold, B.M. Humble and S. Schwarz.
Yield of Immunolabel Compared to Resin Sections and Thawed Cryosections, Y.-D. Stierhof, H. Schwarz, M. Durrenburger, W. Villiger and E. Kellenberger.
Quantitation of Colloidal Gold by Electron Microscopy, T. Kehle and V. Herzog.
Colloidal Gold Labeling for Deteremining Cell Surface Area, V. Herzog and T. Kehle.
Simultaneous Demonstration of Antigens on Outer and Protoplasmic Surfaces of the Plasma Membrane by Replica Immunocytochemistry, G. Rutter and H. Hohenberg.
Double Labeling of Antigenic Sites on Cell Surfaces Imaged with Backscattered Electrons, E. Namork.
Location and Identification of Colloidal Gold Particles with an Energy Dispersive Analyzer, S. Eskelinen and R. Peura.
Use of Dinitrophenol IgG Conjugates: Immunogold Labeling of Cellular Antigens on Thin Sections of Osmicated and Epon-Embedded Specimens, R. Pathak and R.G.W. Anderson.
Preparation and Application of Insulin-Gold Complex, R.M. Smith and L. Jarett.
Microinjection of Colloidal Gold, S.I. Dworetzky.
Combined Diaminobenzidine-Colloidal Gold Staining, H.C.F. Mutasa.
Colloidal Gold Techniques and the Immunocytochemistry of Antigen-Antibody Reactions at Interfaces, H. Nygren and M. Werthen.
Colloidal Gold Labeling for Diagnostic Pathology, G.A. Herrara and R.L. Lott.
Immunogold Silver Staining Using Microwave Irradiation, M.E. Boon, H.J.G. van de Kant, and L.P. Kok.
A CorrelativeApproach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low Voltage Scanning Electron Microscopy, and High Voltage Electron Microscopy, S.L. Goodman, K. Park, and R.M. Albrecht.

Product Details

Principles, Methods, and Applications
Academic Press
Hayat, M.
Hayat, M. A.
Publication Date:
9 x 6.5 in.

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Colloidal Gold: Principles, Method & Application
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Product details pages Academic Press, Incorporated - English 9780123339300 Reviews:
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