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Methods in Molecular Biology #133: Gene Targeting Protocols

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Methods in Molecular Biology #133: Gene Targeting Protocols Cover

 

Synopses & Reviews

Publisher Comments:

In Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting- one using a fusion protein to deliver a vector to a virus for possible therapeutic effect, and one entailing an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may revolutionize the entire field. Innovative and wide ranging, Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding and regulating biological processes at the genetic level.

Book News Annotation:

Kmiec, who discovered the technique known as "chimeraplasty" and currently works at Thomas Jefferson University in Pennsylvania, is joined by researchers from academia and the pharmaceutical industry to present the latest techniques for gene replacement, gene knockout, and gene repair in both plants and animals. They offer several methods for gene targeting, including Cre-ox, and adeno and adeno-associated viruses, as well as the new and potentially revolutionary use of oligonucleotides. This book will provide practical gene-targeting techniques for molecular and cell biologists, geneticists, and gene therapists, as well as biotechnologists involved in genetic manipulation.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Synopsis:

A panel of innovative investigators presents, in readily reproducible detail, the latest techniques for gene replacement, gene knockout, and gene repair in both plants and animals. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting-one uses a fusion protein to deliver a vector to a virus for possible therapeutic effect, and the other entails an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field. Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding biological processes at the genetic level.

Table of Contents

Nucleic Acid Transfer Using Cationic Lipids, Natasha J. Caplen. Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function, Barbara A. Demeneix, Mohamed Ghorbel, and Daniel Goula. Gene Transfer and Drug Delivery by Electronic Pulse Delivery: A Nonviral Delivery Approach, Xi Zhao. Strategies for Improving the Frequency and Assessment of Homologous Recombination, Nancy Smyth Templeton. Effective Gene Transfer Using Viral Vectors Based on SV40, David S. Strayer. Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase, Bruce D. Bethke and Brian Sauer. Site-Directed Alteration of DNA by Small-Fragment Homologous Replacement, Kaarin K. Goncz and Dieter C. Gruenert. Mutation Correction by Homologous Recombination with an Adenovirus Vector, Ayumi Fujita-Kusano, Yasuhiro Naito, Izumu Saito, and Ichizo Kobayashi. Site-Specific Targeting of DNA Plasmids to Chromosome 19 Using AAV Cis and Trans Sequences, Samuel M. Young Jr., Weidong Xiao, and Richard Jude Samulski. Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle, Richard J. Bartlett and Jesica M. McCue. Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vector, Directed Against High-Affinity a-Chain of Human Interleukin-2 Receptor, Sun U. Song and Wayne A. Marasco. EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells, Juliette Fivaz, M. Chiara Bassi, Stéphane Pinaud, Larry Richman, Melanie Price, and Jovan Mirkovitch. Triplex-Directed Site-Specific Genome Modification, Karen M. Vasquez and John H. Wilson. Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylating Oligodeoxynucleotides, Howard B. Gamper, Irina Afonina, Evgeniy Belousov, Michael W. Reed, and Mikhail A. Podyminogin. Gene Targeting in Plants via Site-Directed Mutagenesis, Peter B. Kipp, Joyce M. Van Eck, Peter R. Beetham, and Gregory D. May. Antisense Oligonucleotides as Modulators of Pre-mRNA Splicing, Halina Sierakowska, Sudhir Agrawal, and Ryszard Kole. Index.

Product Details

ISBN:
9780896033603
Editor:
Kmiec, Eric B.
Editor:
Gruenert, Dieter C.
Editor:
Kmiec, Eric B.
Editor:
Gruenert, Dieter C.
Author:
Kmiec, Eric B.
Publisher:
Humana Press
Location:
Totowa, N.J. :
Subject:
Genetics
Subject:
Molecular biology
Subject:
Laboratory Medicine
Subject:
Gene Targeting
Subject:
Life Sciences - Genetics & Genomics
Subject:
Biology-Genetics
Subject:
Human genetics
Edition Description:
Book
Series:
Methods in Molecular Biology Hardcover
Series Volume:
133
Publication Date:
19990931
Binding:
HARDCOVER
Language:
English
Illustrations:
Yes
Pages:
256
Dimensions:
229 x 152 mm 1230 gr

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » Genetics
Science and Mathematics » Chemistry » Chemical Engineering

Methods in Molecular Biology #133: Gene Targeting Protocols New Hardcover
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Product details 256 pages Humana Press - English 9780896033603 Reviews:
"Synopsis" by , A panel of innovative investigators presents, in readily reproducible detail, the latest techniques for gene replacement, gene knockout, and gene repair in both plants and animals. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting-one uses a fusion protein to deliver a vector to a virus for possible therapeutic effect, and the other entails an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field. Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding biological processes at the genetic level.
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