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Methods in Cell Biology #40: A Practical Guide to the Study of Calcium in Living Cells

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Methods in Cell Biology #40: A Practical Guide to the Study of Calcium in Living Cells Cover

 

Synopses & Reviews

Publisher Comments:

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features

* Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes

* fluorescent dyes

* luminescent proteins

* Includes techniques for perturbing intracellular Ca2+

* Covers detailed methodology plus problems and pitfalls of each technique

* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program

* Color plates illustrate techniques such as

* Confocal ratio-imaging

* Use of aequorin

Synopsis:

ions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca2+ buffers appropriate to their research needs.

Highlights

* Describes techniques to

* Detect Ca2+ with sensitive microelectrodes, fluorescent dyes, and luminescent proteins

* Perturb intracellular Ca2+

* Prepare Ca2+ buffers with an easy-to-use computer program

* Contains color plates that illustrate confocal ratio-imaging and the use of aequorin

* Covers methods in detail and describes the problems and pitfalls of each technique

Synopsis:

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features

* Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes

* fluorescent dyes

* luminescent proteins

* Includes techniques for perturbing intracellular Ca2+

* Covers detailed methodology plus problems and pitfalls of each technique

* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program

* Color plates illustrate techniques such as

* Confocal ratio-imaging

* Use of aequorin

Table of Contents

Ca2+ Buffers and [Ca2+]i Perturbation Techniques:

D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers.

R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+.

D. Thomas and M. Hanley, Pharmacological Tools for Perturbing Intracellular Ca2+ Stores.

Microelectrode Techniques for Measuring [Ca2+]i and Ca2+ Fluxes:

S. Baudet, L.H. Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.

P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux.

C. Leech and G.G. Holz, IV, Application of PatchClamp Methods to the Study of Calcium Currents and Calcium Channels

Fluorescence Techniques for Imaging [Ca2+]i:

J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators.

S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.

T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.

P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells.

S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes.

K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]i Using a Photomultiplier Tube.

A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]i with Aequorin Using a Photon Imaging Detector.

R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.

Subject Index.

References.

Product Details

ISBN:
9780125228107
Editor:
Nuccitelli, Richard
Editor:
Matsudaira, Paul T.
Editor:
Wilson, Leslie
Editor:
Nuccitelli, Richard
Editor:
Matsudaira, Paul T.
Author:
Nuccitelli, Richard
Author:
Nuccitelli, Matsudaira, Wilson, Leslie, Paul T., Richard
Author:
Biology, American Society for Cell
Editor:
Wilson, Leslie
Publisher:
Academic Press
Location:
San Diego :
Subject:
General
Subject:
Neuroscience
Subject:
Physiology
Subject:
Cytology
Subject:
Calcium
Subject:
Calcium in the body
Subject:
Calcium channels.
Subject:
Calcium Channels -- analysis.
Subject:
Life Sciences - Biology - General
Subject:
Life Sciences - Biology - Developmental Biology
Subject:
General Medical
Subject:
Biology-General
Edition Description:
Paperback
Series:
Methods in Cell Biology (Paperback)
Series Volume:
G-94-2v. 40
Publication Date:
19940331
Binding:
TRADE PAPER
Language:
English
Illustrations:
Yes
Pages:
368
Dimensions:
9 x 6 in.

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Reference » Science Reference » Technology
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » General
Science and Mathematics » Chemistry » General

Methods in Cell Biology #40: A Practical Guide to the Study of Calcium in Living Cells New Trade Paper
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Product details 368 pages Academic Press - English 9780125228107 Reviews:
"Synopsis" by , ions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca2+ buffers appropriate to their research needs.

Highlights

* Describes techniques to

* Detect Ca2+ with sensitive microelectrodes, fluorescent dyes, and luminescent proteins

* Perturb intracellular Ca2+

* Prepare Ca2+ buffers with an easy-to-use computer program

* Contains color plates that illustrate confocal ratio-imaging and the use of aequorin

* Covers methods in detail and describes the problems and pitfalls of each technique

"Synopsis" by , A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features

* Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes

* fluorescent dyes

* luminescent proteins

* Includes techniques for perturbing intracellular Ca2+

* Covers detailed methodology plus problems and pitfalls of each technique

* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program

* Color plates illustrate techniques such as

* Confocal ratio-imaging

* Use of aequorin

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