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Methods in Cell Biology #70: Cell Biological Applications of Confocal Microscopy

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Methods in Cell Biology #70: Cell Biological Applications of Confocal Microscopy Cover

 

Synopses & Reviews

Publisher Comments:

Cell Biological Applications of Confocal Microscopy is a comprehensive introduction to confocal microscopy for cell biologists. It contains integrated and up-to-date coverage on the various techniques and uses of this valuable research tool. Techniques are described completely and methods are made accessible and understandable, enabling the researcher to study and develop a hands-on approach to imaging. Chapters cover a variety of topics ranging from confocal microscope theory to applications for studies in cell biology. Fundamental topics include discussion on the role of coverglass thickness in 3-D imaging, and selecting a suitable pixel size. More advanced topics include the use of the microscope in ratiometric measurements with physiological dyes, the application of fast video rate microscopes, and the two-photon microscope and its advantages over traditional confocal microscopes. Chapters of interest to developmental and molecular biologists describe the properties of imaging species of animals, the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features

* Includes detailed protocols accessible to new users

* Contains an overview of available confocal microscopes

* Details how to set up and run a "Confocal Microscope Core Facility"

* Contains over 170 figures

Synopsis:

and the two-photon microscope and its advantages over traditional confocal microscopes. Chapters of interest to developmental and molecular biologists describe the properties of imaging species of animals, the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features

* Includes detailed protocols accessible to new users

* Contains an overview of available confocal microscopes

* Details how to set up and run a "Confocal Microscope Core Facility"

* Contains over 170 figures

Synopsis:

the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features

* Includes detailed protocols accessible to new users

* Contains an overview of available confocal microscopes

* Details how to set up and run a "Confocal Microscope Core Facility"

* Contains over 170 figures

Table of Contents

Introduction to Confocal Microscopy.

Direct-View High-Speed Confocal Scanner - the CSU-10; Introduction to Two-Photon Microscopes.

Confocal Microscopy: Important Considerations for Accurate Imaging.

Multicolor-Laser Scanning Confocal Immunofluorescence Microscopy:

Practical Application and Limitations.

Practical Aspects of Objective Lens Selection for Confocal and Multi-Photon Digital Imaging Techniques.

Resolution of Subcellular Detail in Thick Tissue Sections: Immunohistochemical Preparation and Fluorescence Confocal Microscopy.

Confocal Fluorescence Microscopy for Tetrahymena. Confocal Imaging for Drosophila Embryos.

Confocal Fluorescence Microscopy of the Cytoskeleton of Amphibian Oocytes and Embryos. Confocal Fluorescence Microscopy Measurements of pH and Calcium in Living Cells.

Confocal and Non-Linear Optical Imaging of Potentiometric Dyes.

Measurement and Intracellular Ca2+ Concentration. Running and Setting Up a Confocal Microscope Core Facility.

Product Details

ISBN:
9780125804455
Editor:
Matsumoto, Brian
Publisher:
Academic Press
Editor:
Matsumoto, Brian
Author:
Matsumoto, Brian
Location:
San Diego
Subject:
General
Subject:
Cytology
Subject:
Microscopes & Microscopy
Subject:
Microscopy
Subject:
Confocal microscopy
Subject:
Research -- Methodology.
Subject:
Microscopy, Fluorescence.
Subject:
Cytological Techniques.
Subject:
Microscopy, Confocal.
Subject:
Life Sciences - Cytology
Subject:
General Medical
Subject:
Biology-Cytology and Cell Biology
Edition Number:
2
Edition Description:
Paperback
Series:
Methods in Cell Biology (Paperback)
Series Volume:
G-3v. 70
Publication Date:
20021231
Binding:
TRADE PAPER
Language:
English
Illustrations:
Yes
Pages:
507
Dimensions:
9.25 x 7.5 in.

Related Subjects


Reference » Science Reference » General
Reference » Science Reference » Microscopes
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » Genetics
Science and Mathematics » Biology » Molecular
Science and Mathematics » Chemistry » Biochemistry

Methods in Cell Biology #70: Cell Biological Applications of Confocal Microscopy New Trade Paper
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$196.95 Backorder
Product details 507 pages Academic Press - English 9780125804455 Reviews:
"Synopsis" by , and the two-photon microscope and its advantages over traditional confocal microscopes. Chapters of interest to developmental and molecular biologists describe the properties of imaging species of animals, the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features

* Includes detailed protocols accessible to new users

* Contains an overview of available confocal microscopes

* Details how to set up and run a "Confocal Microscope Core Facility"

* Contains over 170 figures

"Synopsis" by , the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features

* Includes detailed protocols accessible to new users

* Contains an overview of available confocal microscopes

* Details how to set up and run a "Confocal Microscope Core Facility"

* Contains over 170 figures

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