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Practical Approach Series #161-: Gene Probes 2: A Practical Approach

by

Practical Approach Series #161-: Gene Probes 2: A Practical Approach Cover

 

Synopses & Reviews

Publisher Comments:

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. The advent of solid supports, new DNA labeling techniques, and PCR technology has created a need for an up-to-date practical manual in this area. Gene Probes (Applications), describes techniques for specific research goals. It covers: hybridization strategies; choosing probes; screening and analysis of DNA libraries, including the use of PCR; blotting techniques, gene targeting; and analysis of human genetic mutations. Its companion volume, Gene Probes 1, describes the wide rage of techniques now available for the purification, synthesis, labeling, detection, and quantitation of probes.

Synopsis:

Gene probes find a variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutation.

Table of Contents

1. Hybridization strategy

2. Screening recombinant DNA libraries by hybridization and amplification

3. Screening human recombinant libraries with nucleic acid probes

4. Antibody screening of bacterial expression systems

5. Isolation of cDNA clones by direct expression in mammalian cells

6. Genomic mapping and gene structure analysis

7. Analysis of gene expression by blotting techniques

8. In situ hybridization of nucleic acid probes to cellular RNA

9. Applications of chromosomal in situ hybridization

10. Analysis of gene expression analysis by PCR: a quantitative approach

11. Analysis of protein expression by immunocytochemistry

12. Gene targeting in mammalian cells

13. Directed mutagenesis and mutant analysis

14. Human gene mutational analysis

Appendix 1

Appendix 2

Product Details

ISBN:
9780199634149
Editor:
Hames, Higgins
Editor:
Hames, B. David
Editor:
Higgins, S. J.
Author:
D, B.
Author:
Higgins, S. J.
Author:
Hames
Author:
null, B. D.
Author:
Hames, Higgins
Author:
null, S. J.
Author:
Hames, B. D.
Author:
Hames, B. David
Publisher:
OUP Oxford
Location:
Oxford ;
Subject:
General
Subject:
Genetics
Subject:
Cell biology
Subject:
DNA probes.
Subject:
Life Sciences - Genetics & Genomics
Subject:
Life Sciences - Cytology
Subject:
Life Sciences | Molecular
Subject:
Life Sciences | Molecular and Cell Biology
Subject:
Life Sciences | Molecular & Cell Biology
Subject:
Biology-Cytology and Cell Biology
Edition Number:
1st ed.
Edition Description:
Includes bibliographical references and indexes.
Series:
Practical approach series ;
Series Volume:
199161-162
Publication Date:
20020331
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
5 color and 56 b/w illus
Pages:
448
Dimensions:
1.38 in.

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Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
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Science and Mathematics » Biology » Molecular
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Practical Approach Series #161-: Gene Probes 2: A Practical Approach New Trade Paper
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Product details 448 pages Oxford University Press - English 9780199634149 Reviews:
"Synopsis" by , Gene probes find a variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutation.
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