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Practical Approach Series #148,: DNA Cloning: A Practical Approach

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Practical Approach Series #148,: DNA Cloning: A Practical Approach Cover

 

Synopses & Reviews

Publisher Comments:

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.

These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Synopsis:

Techniques in molecular biology continue to develop and find wider applications both in research and the biotechnology industry. DNA Cloning aims to extend and complement existing cloning manuals. Contents of Volume I range from the use of phage lamda replacement vectors in the construction of representative genomic DNA libraries to broad host range cloning vectors for Gram-negative bacteria.

Synopsis:

Techniques in molecular biology continue to develop and find wider applications both in research and the biotechnology industry. DNA Cloning aims to extend and complement existing cloning manuals. Contents of Volume I range from the use of phage lamda replacement vectors in the construction of representative genomic DNA libraries to broad host range cloning vectors for Gram-negative bacteria.

Table of Contents

1. Techniques for transformation of E.coli

2. Lambda replacement vectors in the construction of genomic DNA libraries

3. Procedures for cDNA cloning

4. Making probes

5. Using cloned DNAs to analyse gene organization and expression

6. Choices of single-stranded vectors and their application for in vitro mutagenesis

7. PCR and its applications

8. DNA sequencing

Product Details

ISBN:
9780199634767
Editor:
Glover, David M.
Editor:
Hames, B. David
Editor:
Glover, David M.
Author:
Hames, B. D.
Author:
Hames, David Ed. B. Ed. David Ed. B. Ed.
Author:
null, D. M.
Author:
Hames, David Ed. B. Ed. David Ed. B. Ed.
Author:
null, B. D.
Author:
Glover, Hames
Author:
Hames, David
Author:
Glover, D. M.
Author:
Hames, B. David
Publisher:
Oxford University Press, USA
Subject:
Genetics
Subject:
Recombinant DNA
Subject:
Molecular cloning
Subject:
Life Sciences - Cytology
Subject:
Medicine | Genetics
Subject:
Biology-Cytology and Cell Biology
Edition Number:
2
Edition Description:
Second
Series:
Practical Approach Series
Series Volume:
148,
Publication Date:
19950631
Binding:
TRADE PAPER
Grade Level:
Professional and scholarly
Language:
English
Illustrations:
40 illus.
Pages:
288
Dimensions:
6.14x9.21x.60 in. .89 lbs.

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
History and Social Science » Linguistics » General
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » General

Practical Approach Series #148,: DNA Cloning: A Practical Approach New Trade Paper
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Product details 288 pages Oxford University Press - English 9780199634767 Reviews:
"Synopsis" by , Techniques in molecular biology continue to develop and find wider applications both in research and the biotechnology industry. DNA Cloning aims to extend and complement existing cloning manuals. Contents of Volume I range from the use of phage lamda replacement vectors in the construction of representative genomic DNA libraries to broad host range cloning vectors for Gram-negative bacteria.
"Synopsis" by , Techniques in molecular biology continue to develop and find wider applications both in research and the biotechnology industry. DNA Cloning aims to extend and complement existing cloning manuals. Contents of Volume I range from the use of phage lamda replacement vectors in the construction of representative genomic DNA libraries to broad host range cloning vectors for Gram-negative bacteria.
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