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Chromatin: A Practical Approach (Practical Approach Series)

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Chromatin: A Practical Approach (Practical Approach Series) Cover

 

Synopses & Reviews

Publisher Comments:

Developments in the analysis of the regulation of chromatin structure have provided much information on their relevance in the control of DNA replication and transcription, making it an important area of research. Chromatin: A Practical Approach provides clear explanations of a wide range of techniques from the initial isolation and fractionation of chromatin and linker histones, to the in vitro assembly of chromatin and nuclear structures in Xenopus egg extracts. Both in vivo and in vitro methods are covered and clear explanations of procedures are provided throughout making this volume an invaluable aid to laboratory workers in biochemistry and molecular biology, especially those whose research encompasses eukaryotic transcription, DNA replication, and chromosome structure.

Table of Contents

1. Isolation and fractionation of chromatin and linker histones

2. Extraction and separation of core histones and non-histone chromosomal proteins

3. Mapping chromatin structure in Drosophila

4. In vivo footprint and chromatin analysis by LMPCR

5. Analysis of nuclear scaffold attachment regions

6. Electron microscopy of chromatin

7. Counting nucleosome cores on circular DNA using topoisomerase I

8. In vitro reconstitution of chromatin and analysis of nucleosome positioning

9. Transcriptional and structural analysis of chromatin assembled in vivo

10. An assembly of chromatin and nuclear structures in Xenopus egg extracts

Appendix: List of Suppliers

Index

Product Details

ISBN:
9780199635986
Editor:
Gould, Hannah
Author:
Gould, H.
Author:
null, H.
Author:
Gould, Hannah
Author:
Gould, Hannah Flagg
Author:
Gould
Publisher:
OUP Oxford
Location:
Oxford ;
Subject:
Biochemistry
Subject:
Methodology
Subject:
Research
Subject:
Laboratory Medicine
Subject:
Life Sciences - Biochemistry
Subject:
Chromatin -- Laboratory manuals.
Subject:
Chromatin -- Research -- Methodology.
Subject:
Chemistry-Biochemistry
Edition Description:
Includes bibliographical references and index.
Series:
Practical Approach Series
Series Volume:
189
Publication Date:
19980431
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
, s
Pages:
256
Dimensions:
9.100 x 6.100 in 0.950 lb

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Chemistry » Biochemistry
Science and Mathematics » Chemistry » Chemical Engineering

Chromatin: A Practical Approach (Practical Approach Series) New Trade Paper
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Product details 256 pages Oxford University Press - English 9780199635986 Reviews:
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