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Practical Approach Series #186: PCR 3: PCR in Situ Hybridization: A Practical Approach

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Practical Approach Series #186: PCR 3: PCR in Situ Hybridization: A Practical Approach Cover

 

Synopses & Reviews

Publisher Comments:

PCR 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

Table of Contents

1. Fixation of tissues for the polymerase chain reaction

2. In situ genetic analysis with selective ultraviolet radiation fractionation (SURF)

3. In situ hybridization

4. PCR in situ hybridization (PCR-ISH)

5. Reverse transcriptase in situ PCR for RNA detection

6. Primed in situ DNA synthesis (PRINS)

7. Application of in situ-PCR techniques to human tissues

8. Applications and modifications of PCR in situ hybridization

9. Automation of in situ amplification

Product Details

ISBN:
9780199636327
Editor:
O'Leary, John J.
Editor:
Herrington, C. Simon
Editor:
O'Leary, John J.
Author:
null, C. Simon
Author:
Herrington, O'Leary
Author:
null, John J.
Author:
O'Leary, John J.
Author:
Herrington, C. Simon
Publisher:
OUP Oxford
Location:
Oxford ;
Subject:
Genetics
Subject:
Polymerase chain reaction
Subject:
Life sciences
Subject:
In situ hybridization
Subject:
Life Sciences - Genetics & Genomics
Subject:
Life Sciences - Biochemistry
Subject:
Life Sciences | Molecular
Subject:
Cell Biology | Developmental Biology
Subject:
Life Sciences | Mole
Subject:
cular
Subject:
Life Sciences | Molecular and Cell Biology | Developmental Biology
Subject:
In situ hybridization -- Laboratory manuals.
Subject:
Life Sciences | Molecular & Cell Biology | Developmental Biology
Subject:
Health and Medicine-Medical Specialties
Subject:
| Molecular & Cell Biology | Developmental Biology
Edition Number:
2
Edition Description:
Includes bibliographical references and index.
Series:
Practical approach series ;
Series Volume:
186
Publication Date:
19971031
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
55 illus.
Pages:
224
Dimensions:
71 in.

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Genetics
Science and Mathematics » Biology » Molecular
Science and Mathematics » Chemistry » Biochemistry
Textbooks » General

Practical Approach Series #186: PCR 3: PCR in Situ Hybridization: A Practical Approach New Trade Paper
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Product details 224 pages Oxford University Press - English 9780199636327 Reviews:
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