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Practical Approach Series #188: Mutation Detection: A Practical Approach

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Practical Approach Series #188: Mutation Detection: A Practical Approach Cover

 

Synopses & Reviews

Publisher Comments:

Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.

Synopsis:

Mutation detection is increasingly undertaken in a wide spectrum of research areas. This volume offers tried and tested protocols for a range of detection methods, from the labs of researchers in the field.

Table of Contents

1. Single strand conformation polymorphism analysis

2. Single-stranded conformation polymorphism and heteroduplex analysis

3. Comprehensive mutation detection with denaturing gradient gel electrophoresis

4. Cleavage using RNase to detect mutations

5. Cleavage of mismatched bases using chemical reagents

6. Mutation detection using T4 endonuclease VII

7. Detection of mutations by hybridization with sequence-specific oligonucleotide probes

8. DNA detection and sequence distinction through oligonucleotide ligation

9. Detection of sequence variation using primer extension

10. Selective amplification of specific alleles

11. The protein truncation text (PTT)

12. Functional assay of the p53 tumour suppressor gene

13. Advances in direct DNA sequencing for mutation scanning

Product Details

ISBN:
9780199636563
Editor:
Cotton, R. G.
Editor:
Forrest, S.
Editor:
Cotton, R. G.
Author:
E. Edkins
Author:
Edkins, Forrest Cotton
Author:
R. G. H. Cotton
Author:
Forrest, S.
Publisher:
OUP Oxford
Location:
Oxford ;
Subject:
Genetics
Subject:
Molecular genetics
Subject:
Mutation (Biology)
Subject:
Life Sciences - Genetics & Genomics
Subject:
Life Sciences - Evolution
Subject:
Life Sciences | Genetics
Subject:
Mutation (Biology) -- Laboratory manuals.
Subject:
Molecular genetics -- Laboratory manuals.
Subject:
Health and Medicine-Medical Specialties
Edition Number:
2
Edition Description:
Includes bibliographical references and index.
Series:
Practical Approach Series
Series Volume:
188
Publication Date:
19980231
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
66 illus.
Pages:
264
Dimensions:
83 in.

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Evolution
Science and Mathematics » Biology » Genetics
Science and Mathematics » Biology » Molecular

Practical Approach Series #188: Mutation Detection: A Practical Approach New Trade Paper
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Product details 264 pages Oxford University Press - English 9780199636563 Reviews:
"Synopsis" by , Mutation detection is increasingly undertaken in a wide spectrum of research areas. This volume offers tried and tested protocols for a range of detection methods, from the labs of researchers in the field.
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