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Practical Approach Series #244: Protein Purification Techniques: A Practical Aproach

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Practical Approach Series #244: Protein Purification Techniques: A Practical Aproach Cover

 

Synopses & Reviews

Publisher Comments:

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

Book News Annotation:

It's been ten years since publication of the first edition of this text. The second edition provides the most recent strategies and detailed practical procedures for laboratory-scale protein purification from common sources. Coverage includes fusion methods; initial purification of inclusion bodies; purification of crystallography; and purification from mammalian cell culture, microbial cell culture, milk, animal tissue, and plant sources.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Synopsis:

The core of this text is the provision of detailed practical guidelines aimed particularly at laboratory scale purification of proteins. Information on scale-up considerations is given where appropriate.

Table of Contents

List of protocols

Abbreviations

1. Purification strategy, Simon D. Roe

2. Getting started, Simon D. Roe

3. Analysis of purity, Dev Baines

4. Clarification techniques, Ian Reed and Duncan Mackay

5. Cell disintegration and extraction techniques, R. H. Cumming and G. Iceton

6. Concentration of the extract, E. L. V. Harris

7. Separation on the basis of chemistry, Lars Hagel

8. Chromatography on the basis of size, P. Cutler

9. Purification by exploitation of activity, S. Angal, P. D. G. Dean, and Simon D. Roe

10. Scale-up considerations, John B. Noble

A1. List of suppliers

Index

Product Details

ISBN:
9780199636730
Editor:
Roe, Simon
Publisher:
Oxford University Press, USA
Editor:
Roe, Simon
Author:
Roe, Simon
Author:
null, Simon
Location:
Oxford
Subject:
Biochemistry
Subject:
Purification
Subject:
Proteins
Subject:
Laboratory Medicine
Subject:
Enzymology
Subject:
Research -- Methodology.
Subject:
Life Sciences - Biochemistry
Subject:
Life Sciences | Biochemistry | Proteins
Subject:
Life Sciences | Biochemistry | Proteins and Enzymology
Subject:
Proteins -- Purification.
Subject:
Life Sciences | Biochemistry | Proteins & Enzymology
Subject:
Chemistry-Biochemistry
Copyright:
Edition Number:
2
Edition Description:
Second
Series:
The practical approach series
Series Volume:
244
Publication Date:
20010431
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
2 halftones and 37 line illus
Pages:
280
Dimensions:
9.83x7.55x.63 in. 1.11 lbs.

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Reference » Science Reference » General
Reference » Science Reference » Philosophy of Science
Science and Mathematics » Biology » General
Science and Mathematics » Biology » Molecular
Science and Mathematics » Chemistry » Biochemistry

Practical Approach Series #244: Protein Purification Techniques: A Practical Aproach Used Trade Paper
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Product details 280 pages Oxford University Press - English 9780199636730 Reviews:
"Synopsis" by , The core of this text is the provision of detailed practical guidelines aimed particularly at laboratory scale purification of proteins. Information on scale-up considerations is given where appropriate.
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