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Gene Targeting: A Practical Approach (Practical Approach Series)

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Synopses & Reviews

Publisher Comments:

Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.

Synopsis:

This updated and revised edition is well illustrated with over 60 figures and nearly 20 photographs. With over 50 protocols, it is useful for postgraduate engineers, molecular biologists, and biomedical researchers.

Table of Contents

Preface

List of Contributors

Abbreviations

1. Gene targeting, principles, and practice in mammalian cells, Paul Hasty, Alejandro Abuin, and Allan Bradley

2. Site-specific recombination in cell and mice, Susan M. Dymecki

3. Production of targeted embryonic stem cell clones, Michael P. Matise, Wotjek Auerbach, and Alexandra L. Joyner

4. Production of chimeras by blastocyst and morula injection of targeted ES cells, Virginia Papaioannou and Randall Johnson

5. Production and analysis of ES cell aggregation chimeras, Andras Nagy and Janet Rossant

6. Gene trap strategies in ES cells, Wolfgang Wurst and Achim Gossler

7. Classical genetics and gene targeting, Scott Bultman and Terry Magnuson

List of suppliers

Index

Product Details

ISBN:
9780199637928
Other:
Joyner, Alexandra L.
Publisher:
Oxford University Press, USA
Editor:
Joyner, Alexandra L.
Author:
Joyner, Alexandra L.
Author:
Joyner, A. L.
Author:
null, Alexandra L.
Subject:
Mammals
Subject:
Molecular biology
Subject:
Biotechnology
Subject:
Laboratory Medicine
Subject:
Gene Targeting
Subject:
Life Sciences | Genetics
Subject:
Animals - Mammals
Subject:
Nature Studies-Mammals
Edition Number:
2
Edition Description:
Second
Series:
Practical Approach Series
Series Volume:
212
Publication Date:
20000331
Binding:
TRADE PAPER
Grade Level:
College/higher education:
Language:
English
Illustrations:
96 halftones, and line illus
Pages:
312
Dimensions:
6.1 x 9.1 x 1 in 1.106 lb

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » General
Science and Mathematics » Biology » Genetics
Science and Mathematics » Chemistry » Chemical Engineering
Science and Mathematics » Nature Studies » Mammals » General

Gene Targeting: A Practical Approach (Practical Approach Series) New Trade Paper
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Product details 312 pages Oxford University Press - English 9780199637928 Reviews:
"Synopsis" by , This updated and revised edition is well illustrated with over 60 figures and nearly 20 photographs. With over 50 protocols, it is useful for postgraduate engineers, molecular biologists, and biomedical researchers.
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