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Other titles in the Practical Approach Series series:
Practical Approach Series #250: Plant Cell Biology: A Practical Approachby Chris Hawes
Synopses & Reviews
With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
Book News Annotation:
Surveys techniques for studying the location and function of proteins within a cell, with an emphasis on microscopy. The 13 chapters introduce fluorescent probes, cytometry, microinjection, micromanipulation, the isolation of cytoplasmic organelles, and classical histochemistry. The second edition adds chapters on the expression of heterologous proteins in protoplasts and the green fluorescent protein.
Annotation c. Book News, Inc., Portland, OR (booknews.com)
Table of Contents
List of protocols
1. Introduction to optical microscopy for plant cell biology, P.J. Shaw
2. Fluorescent probes for living plant cells, Mark Fricker, et al.
3. Flow cytometry, Teodoro Coba de la Ped~na and Spencer Brown
4. Transient expression, a tool to address questions in plant cell biology, Jane L. Hadlington and Jürgen Denecke
5. The green fluorescent protien (GFP) as reporter in plant cells, Jean-Marc Neuhaus and Petra Boevink
6. Microinjection, Michael Knoblauch
7. Micromanipulation by laser microbeam and optical tweezers, Karl Otto Greulich
8. Electrophysiological methods: moitoring exo- and endocytosis in real time, Gerhard Thiel, Jens-Uwe Sutter, and Ulrike Homann
9. Plant histology, Jackie Spence
10. Immunocytochemistry from light microscopy, Béatrice Satiat-Jeunemaitre and Chris Hawes
11. Electron Microscopy, Chris Hawes and Béatrice Satiat-Jeunemaitre
12. In situ hybridization, A.R. Leitch, et al.
13. Organelle isolation, David G. Robinson and Giselbert Hinz
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Science and Mathematics » Biology » Cytology and Cell Biology