Insect Cell Culture: Fundamental & Applied Aspects

Insect Cell Cultures: Fundamental and Applied Aspects is a comprehensive reference work, covering the key issues involved in nearly 30 review papers: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; economics and regulatory aspects. For cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.

Contains some 30 mini-reviews on recent developments in all scientific disciplines involved with insect cell culture technology. Five sections review basic insect cell culture and cell biology, survey the molecular biology of baculoviruses, examine the application of bioengineering principles for the scale-up of insect cell culture, detail examples of commercial products produced in insect cell systems, and discuss economic and regulatory issues. For students and researchers, especially industrial scientists applying baculovirus- cell culture systems for commercial purposes.
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Preface. Development and characterization of insect cell lines; D. Lynn. New approaches to insect tissue culture; D. Baines. Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene; A.M. Fallon. Insect cell physiology; R. Bhatia. Insect cell cultivation: growth and kinetics; G. Schmid. Medium design for insect cell culture; E.-J. Schlaeger. Baculovirus-insect interactions; G.W. Blissard. Replication patterns and cytopathology of cells infected with baculoviruses; G.V. Williams, P. Faulkner. Construction of baculovirus recombinants; P.A. Kitts. Passage effect of virus infection in insect cells; P.J. Krell. Post-translational modifications in insect cells; H.-D. Klenk. Productivity of insect cells for recombinant proteins; J.E. Maruniak. Chaperone and foldase coexpression in the baculovirus-insect cell expression system; M.J. Betenbaugh, et al. Shear sensitivity of insect cells; J.J. Chalmers. Insect cell bioreactors; S.N. Agathos. Perfusion for the production of recombinant proteins in insect cells; V. Jäger. Immobilization of insect cells; Jian-Yong Wu, M.F.A. Goosen. Modelling baculovirus infection of insect cells in culture; J.F. Power, L.K. Nielsen. Scale up aspects of sparged insect-cell bioreactors; J. Tramper, et al. Oxygen gradients in small and big sparged insect-cell bioreactors; J. Tramper, et al. Downstream processing of insect cell cultures; A.R. Bernard, et al. Parvovirus diagnostics and vaccine production in insect cells; J.I. Casal. Classical swine fever virus diagnostic and vaccine production in insect cells; M.M. Hulst, R.J.M. Moormann. Production of multidomain complement glycoproteins in insect cells; P. Závodski, S. Cseh. Economics of baculovirus&endash;insect cell production systems; D.J. Rhodes. Safety aspects of insect cell culture; G. Stacey. Regulatory issues in the use of insect-cell culture; F.L.J. van Lier. Keyword index.