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Methods in Molecular Biology #07: Gene Transfer and Expression Protocols

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Methods in Molecular Biology #07: Gene Transfer and Expression Protocols Cover

 

Synopses & Reviews

Publisher Comments:

Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single technique. An Introduction explains the significance of the protocol and provides background information. A Materials section lists all the requirements for the technique discussed. A Methods section details the procedure in a step-by-step protocol. A Notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. Each technique is designed to guarantee optimum results. This volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. Topics and techniques include: viral vectors • reporter genes • analysis of steady-state level of transcription • assay for newly initiated transcriptional complexes • immunocytological techniques • assay of structure and replication state of transfected genes • overall strategy for defining regulatory sequences Providing the latest techniques in a fast-paced area, Gene Transfer and Expression Protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.

Product Details

ISBN:
9780896031784
Author:
Murray, E. J.
Publisher:
Humana Press
Author:
Murray, Edward J.
Author:
Murray
Subject:
General
Subject:
Technique
Subject:
Gene expression
Subject:
Life Sciences - Cytology
Subject:
Genetics
Subject:
Life Sciences - Genetics & Genomics
Subject:
Life Sciences - Biology - Molecular Biology
Subject:
Genetic transformation -- Technique.
Subject:
Transfection -- Technique.
Subject:
Health and Medicine-Medical Specialties
Subject:
Human genetics
Edition Description:
Book
Series:
Methods in Molecular Biology
Series Volume:
07
Publication Date:
19910431
Binding:
HARDCOVER
Language:
English
Illustrations:
Y
Pages:
454
Dimensions:
10.10x7.20x1.10 in. 2.15 lbs.

Related Subjects

Children's » Activities » General
Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » Molecular
Science and Mathematics » Biology » Zoology » General
Science and Mathematics » Chemistry » Chemical Engineering
Science and Mathematics » Physics » Nuclear

Methods in Molecular Biology #07: Gene Transfer and Expression Protocols New Hardcover
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Product details 454 pages Humana Press - English 9780896031784 Reviews:
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