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Protocols in Molecular Parasitology

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Protocols in Molecular Parasitology Cover

 

Synopses & Reviews

Publisher Comments:

Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.

Book News Annotation:

The study of parasitic organisms--particularly those responsible for major diseases such as malaria, schistosomiasis, and trypanosomiasis--has been propelled by advances in recombinant DNA technology and, over the last decade, considerable progress has been made in understanding these organisms at the molecular level. This volume presents a collection of 34 readily reproducible protocols describing techniques proven to be of major importance in the emerging field of molecular parasitology. Chapters are contributed by leading figures who have either developed, or are especially familiar with, each technique. Plastic comb binding.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Table of Contents

Culturing and Biological Cloning of T. brucei. Culturing and Biological Cloning of T. cruzi. In Vitro Cultivation and Biological Cloning of Leishmania. Simple In Vitro Cultivation of the Malaria Parasite P. falciparum (Erythrocytic Stages) Suitable for Large-Scale Preparations. Synchronization and Cloning of Malaria Parasites. The Culture and Prparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies. The Culture of S. mansoni and Production of Life Cycle Stages. Preparation of DNA and RNA from T. brucei. Isolation of RNA and DNA from T. cruzi. Isolation of DNA and RNA from Leishmania. The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite P. falciparum. Extraction and Purificaiton of Nucleic Acids from Schistosomes. Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting. The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification. Analysis of the DNA of Parasitic Protozoa by Flow Cytometry. PCR for Low-Level Detection of Malaria Parasites in Blood. PCR Methods for Identification of Point Mutations and Gene Rearrangements. Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques. Ribosomal RNA Probes for Detection and Identification of Species. Rapid Sequencing of Parasite rRNA. Antibody Screening of Expression Libraries. Antibody Select Procedure for Characterization of Expression Clones. Isolation of Parasite Genes Using Synthetic Oligonucleotides. Chromosome Mapping Methods for Parasitic Protozoa. Transfection of Leishmania and Trypanosoma brucei by Electroporation. Two-Dimensional Polyacrylamide Gel Electrophoresis. Isoenzyme Electrophoresis for Parasiste Characterization. The Surface Labeling of Schistosomes. Monoclonal Antibody Affinity Chromatography. Immunoblotting and Enzyme Linked Immunosorbent Assay. Immunofluorescence of Parasites. Immunoelectron Microscopic Localization of Antigens in Malaria Parasites. Preparation of Blotted Membrane for Protein Microsequencing. Identification of T-Cell Epitopes. Index.

Product Details

ISBN:
9780896032392
Author:
Hyde, John E.
Publisher:
Humana Press
Location:
Totowa, N.J. :
Subject:
General
Subject:
Molecular biology
Subject:
Parasitology
Subject:
Parasites
Subject:
Molecular parasitology.
Subject:
Life Sciences - Ecology
Subject:
Microbiology
Subject:
Life Sciences - Biology - Microbiology
Subject:
Life Sciences - Biology - Molecular Biology
Subject:
Research -- Methodology.
Subject:
Health and Medicine-Medical Specialties
Edition Description:
Book
Series:
Methods in Molecular Biology
Series Volume:
v. 21
Publication Date:
19930831
Binding:
TRADE PAPER
Language:
English
Illustrations:
Yes
Pages:
484
Dimensions:
235 x 155 mm 1 gr

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Protocols in Molecular Parasitology New Trade Paper
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