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Other titles in the Methods in Molecular Biology series:
Chromosome Analysis Protocolsby John R. Gosden
Synopses & Reviews
Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.
Book News Annotation:
Provides step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but most of the techniques are applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes from a number of different cell types, and techniques for producing bonded chromosomes and analyzing karyotypes. Numerous molecular techniques for analyzing chromosomes directly at the light- microscopic level, and other molecular genetics methods are described by scientists who regularly use them in their laboratories. Plastic comb binding.
Annotation c. Book News, Inc., Portland, OR (booknews.com)
Table of Contents
Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and Banding. Chromosome Banding and Identification: Absorption Staining. Chromosome Banding and Identification: Fluorescence. Chromosome Banding: Stain Combinations for Specific Regions. Inhibition of Chromosome Condensation. Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity. Automated Karyotype Analysis. Bivariate Chromosome Analysis Using a Commercial Flow Cytometer. Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping. Electrophoretic Karyotype Analysis: Pulse Field Gel Electrophoresis. Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations. Immunocytochemical Techniques Applied to Meiotic Chromosome Preparations. Immortalized Cell Lines: Their Creation and Use in Gene Mapping. Oligonucleotide-Primed In Situ DNA Synthesis: An Alternative to In Situ Hybridization for Gene Mapping and the Investigation of Genome Organization. Electron Microscopic Localization of In Situ Hybrids. Protocols for Chromosome Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping. Construction of Chromosome Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines. Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints. Chromosome Dissection and Cloning. Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization. Molecular Analysis of Chromosome Aberrations: In Situ Hybridization. Alu- and L1-Primed PCR-Generated Probes for Non-Isotopic In Situ Hybridization. Chromosome Substructure Investigation: Telomeres. Index.
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