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Protocols for Oligonucleotides & Analogs: Synthesis & Propertiesby Sudhir Agrawal
Synopses & Reviews
Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.
Book News Annotation:
Active researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The 19 protocols presented cover all possible DNA analogs that have been studied to date. Each protocol includes an introduction, a bill of material (including suppliers), detailed step-by-step instructions, and notes for avoiding pitfalls and achieving the best results. Plastic comb binding.
Annotation c. Book News, Inc., Portland, OR (booknews.com)
Table of Contents
A Brief History of Oligonucleotide Synthesis. Oligonucleotide Synthesis: Phosphotriester Approach. Oligodeoxyribonucleotide Synthesis: Phosphoramidite Approach. Oligodeoxynucleotide Synthesis: H-Phosphonate Approach. Oligoribonucleotide Synthesis: The Silyl-Phosphoramidite Method. Synthesis of 2'-O-Alkyloligoribonucleotides. An Improved Method for the Synthesis and Deprotection of Methylphosphonate Oligonucleotides. Oligonucleotide Phosphorothioates. Synthesis and Purification of Phosphorodithioate DNA. Oligodeoxyribonucleotide Phosphotriesters. Oligonucleotide Boranophosphate (Borane Phosphate). Oligonucleotide Phosphorofluoridates and Fluoridates. a-Oligonucleotides. Stereospecific Synthesis of P-Chiral Analogs of Oligonucleotides. Oligonucleotide Analogs with Dimethyl-Sulfide, -Sulfoxide, and -Sulfone Groups Replacing Phosphodiester Linkages. Oligonucleotide Analogs Containing Dephosphointernucleoside Linkages. Scaleup of Oligonucleotide Synthesis: Solution Phase. Large Scale Oligonucleotide Synthesis Using Solid Phase Approach. Solid-Phase Supports in Oligonucleotide Synthesis. Index.
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