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This title in other editions

Basic Protein & Peptide Protocols

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Basic Protein & Peptide Protocols Cover

 

Synopses & Reviews

Publisher Comments:

Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!

Book News Annotation:

An update to Walker's classic Proteins (1984), adding many new tested basic laboratory methods for protein and peptide analysis, for both those new to the protein chemistry laboratory and more established workers who wish to try a new technique for the first time. Among the key protocols included are protein and peptide electrophoresis techniques, gel staining methods, methods for protein quantitation, peptide mapping, protein blotting and various detection systems, peptide sequencing, glycoprotein analysis, production of polyclonal and monoclonal antibodies, ELISA, the use of immobilized pH gradients in isoelectric focusing, IgG purification, the use of immunogold reagents, and the detection and analysis of thiol groups. Plastic comb binding.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Table of Contents

Lowry method for protein quantitation — Bicinchoninic acid (BCA) assay for protein quantitation — Bradford method for protein quantitation — Nondenaturing polyacrylamide gel electrophoresis of proteins — SDS polyacrylamide gel electrophoresis of proteins — Gradient SDS polyacrylamide gel electrophoresis of proteins — Acetic acid-urea polyacrylamide gel electrophoresis of proteins — Electrophoresis of peptides — Isoelectric focusing of proteins in ultra-thin polyacrylamide gels — In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis — Two-dimensional polyacrylamide gel electrophoresis of proteins — Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension.

Product Details

ISBN:
9780896032699
Editor:
Walker, John M.
Publisher:
Humana Press
Editor:
Walker, John M.
Author:
Walker
Author:
Walker, John M.
Location:
Totowa, N.J. :
Subject:
General
Subject:
Proteins
Subject:
Peptides
Subject:
Peptides -- Analysis -- Methodology.
Subject:
Life Sciences - Biochemistry
Subject:
Proteins -- Analysis -- Methodology.
Subject:
Biochemistry
Subject:
Biochemistry, general
Subject:
Health and Medicine-General
Copyright:
Edition Description:
Book
Series:
Methods in Molecular Biology (Paperback)
Series Volume:
v. 32
Publication Date:
19940426
Binding:
TRADE PAPER
Language:
English
Illustrations:
Yes
Pages:
502
Dimensions:
235 x 155 mm 1 gr

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Basic Protein & Peptide Protocols New Trade Paper
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Product details 502 pages Humana Press - English 9780896032699 Reviews:
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