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Elisa: Theory and Practice

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Synopses & Reviews

Publisher Comments:

ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation.

ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

Book News Annotation:

Introduces to scientists at all levels of expertise the principles of the most commonly used assay technique, known as Enzyme Linked Immunoabsorbent Assay. Provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in current science, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. Plastic comb- binding.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Synopsis:

ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

Table of Contents

Basic Immunology: 1. The Immune Response 2. Antigens 3. Adaptive Immunity and Clonal Selection 4. Antibodies 5. Antigenic Commonness Basic Principles of ELISA: 1. Reaction Schemes 2. Direct ELISA 3. Indirect ELISA 4. Sandwich ELISA 5. Competition ELISA 6. Choice of Assays Stages in ELISA: 1. Solid-Phase 2. Washing 3. Addition of Reagents 4. Incubation 5. Blocking Conditions and Nonspecific Reactions 6. Enzyme Conjugates 7. Availability of Conjugates 8. Conjugation with Enzymes 9. Development of Label 10. Stopping Reactions 11. Reading 12. Practical Problems Theoretical Considerations: 1. Setting Up and Use of ELISA 2. What Is Already Known 3. Complexity of Problems 4. Antigenic Considerations 5. Other Techniques 6. Units 7. Making Dilutions 8. Pipets 9. Molarities Direct ELISA: Titration of Antigen and Antibody: 1. Learning Principles 2. Reaction Scheme 3. Basis of Assay 4. Materials and Equipment 5. Practical Details 6. Data Explained 7. Aspects of Assay Described 8. Conclusions Indirect ELISA: 1. Learning Principles 2. Basis of Assay 3. Materials and Reagents 4. Practical 5. Use of Indirect ELISA to Titrate Antibodies 6. Use of Indirect ELISA to Determine the Positivity of Sera at Single Dilution Use of Antibodies on Solid-Phase in Capture ELISA: 1. Use of Capture ELISA to Detect and Titrate Antigen 2. Reaction Scheme 3. Basis of Assay 4. Materials 5. Methods 6. Titration of Capture Antibody Using IgG 7. Titration of Capture Antibody when Used as Whole Serum 8. Use of Capture ELISA to Detect Antigens 9. Use of Capture ELISA to Detect and Titrate Antibodies Competitive ELISA: 1. General Information 2. Direct Competitive ELISA for Antigen Detection and Quantification 3. Indirect Assay-Antigen Competition 4. Indirect Competition Assay for Antibody Detection 5. Indirect Assay Competition for Antibody-Detection Using a Single Dilution of Test Serum 6. Overall Conclusions on Competition Assays Immunochemical Techniques: 1. Introduction 2. Labeling Antibodies with Enzymes 3. Coupling Antibodies to Alkaline Phosphatase 4. Preparation of Immunoglobulins 5. Immunosorbents 6. Production of Antisera

Product Details

ISBN:
9780896032798
Author:
Crowther, John R.
Author:
Crowther, J. R.
Author:
Crowther, John R.
Publisher:
Humana Press
Location:
Totowa, N.J. :
Subject:
Reference
Subject:
Immunology
Subject:
Molecular biology
Subject:
Clinical Chemistry
Subject:
Enzymes
Subject:
Immunoassay
Subject:
Enzyme-linked immunosorbent assay.
Subject:
Life Sciences - Biology - Molecular Biology
Subject:
Health and Medicine-Professional Medical Reference
Edition Description:
Book
Series:
Methods in Molecular Biology Paperback
Series Volume:
28
Publication Date:
19950531
Binding:
TRADE PAPER
Language:
English
Illustrations:
Yes
Pages:
238
Dimensions:
229 x 152 mm 760 gr

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Health and Self-Help » Health and Medicine » Professional Medical Reference
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Elisa: Theory and Practice New Trade Paper
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Product details 238 pages Humana Press - English 9780896032798 Reviews:
"Synopsis" by , ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
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