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Practical Molecular Virology

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Practical Molecular Virology Cover

 

Synopses & Reviews

Publisher Comments:

Mary K. L. Collins has assembled in Practical Molecular Virology a vanguard collection of readily repeatable methods for gene transfer and expression using a variety of recombinant viral vectors. In keeping with the established tradition of the series, each technique is presented in an easy-to-follow format designed to work for the novice as well as the seasoned expert. Chapters cover: • life cycles of specific retroviruses and how recombinant vectors are constructed • PCR techniques • poliovirus vectors • herpesvirus vectors • syncytial assays • cell lineage studies • baculovirus and adenovirus vectors • SV40 and EBV vectors • viruses in gene transfer to eukaryotic cells The wealth of material devoted to recombinant retroviral methods and their applications make Practical Molecular Virology and extremely timely volume, one that will find widespread use throughout biological and biomedical research.

Table of Contents

The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors. Construction of Retroviral Packaging Cell Lines. Making High Titer Retroviral Producer Cells. Methods of Titrating Nonselectable Recombinant Retroviruses. Selectable Markers for Eukaryotic Cells. Virus Genome Detection by the Polymerase Chain Reaction (PCR). Synctial Assays. Neutralization of Virus. Vesicular Stomatitis Virus Pseudotypes of Retroviruses. Cell Lineage Studies Using Retroviruses. Retroviral Vectors as Insertional Mutagents. Assessment of Compounds for Anti-HIV Activity. Analysis of Protein Factors that Interact with Adenovirus Early Promoters. COS Cell Expression. Papillomaviruses and Assays for Transforming Genes. Herpes Simplex Virus Life Cycle and the Design of Viral Vectors. Growth of Herpes Simplex Virus and Purification of Viral DNA. Vectors Based on Epstein-Barr Virus. Immortalization of Human B-Lymphocytes by Epstein-Barr Virus. Vaccinia Virus as an Expression Vector. Isolation and Handling of Recombinant Vaccinia Viruses. Design and Construction of Poliovirus Epitope Expression Vectors. Growth and Characterization of Poliovirus Antigen Chimeras. Expression Vectors for the Construction of Hybrid Ty-VLPs. Production and Purification of Hybrid Ty-VLPs. Baculovirus Expression Vectors: Choice of Expression Vector. Baculovirus Expression Vectors: Generating a Recombinant Baculovirus. Baculovirus Expression Vectors: Scale-Up and Downstream Processing. Index.

Product Details

ISBN:
9780896032996
Author:
Collins, Mary K. L.
Publisher:
Humana Press
Author:
Collins, Mary K.
Subject:
General
Subject:
Life Sciences - Biology - Molecular Biology
Subject:
Life Sciences - Cytology
Subject:
Cytology
Subject:
Cell biology
Subject:
Health and Medicine-General
Subject:
Molecular biology
Copyright:
Edition Description:
Book
Series:
Methods in Molecular Biology
Series Volume:
8
Publication Date:
19911031
Binding:
TRADE PAPER
Language:
English
Pages:
340
Dimensions:
235 x 155 mm 1120 gr

Related Subjects

Health and Self-Help » Health and Medicine » General
Health and Self-Help » Health and Medicine » General Medicine
Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Cytology and Cell Biology
Science and Mathematics » Biology » Microbiology
Science and Mathematics » Biology » Molecular

Practical Molecular Virology New Trade Paper
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Product details 340 pages Humana Press - English 9780896032996 Reviews:
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