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Other titles in the Methods in Molecular Biology series:
Methods in Molecular Biology #47: Electroporation Protocols for Microorganismsby Jac A. Nickoloff
Synopses & Reviews
Electroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research. It includes reproducible protocols for diverse bacterial, fungal, and protist species-many of which are important in human disease-as well as literature references to electroporation protocols for related species. The contributors also discuss electroporation theory and instrumentation, making it possible to develop new protocols or modify existing ones, and they provide extensive details about culturing and storing many species in a manner designed to optimize electroporation efficiency. Electroporation Protocols for Microorganisms is an indispensable resource for molecular geneticists working directly with microorganisms and for those who employ microorganisms to prepare materials for later introduction into higher organisms, such as plants and animals. Two companion volumes will follow: Plant Cell Electroporation and Electrofusion Protocols and Animal Cell Electroporation and Electrofusion Protocols.
Book News Annotation:
A laboratory manual for the technique widely used in molecular genetics to introduce DNA into cells for investigations of gene structure and function, and increasingly used for other research purposes such as introducing other types of biological and analytic molecules into cells, to induce cell-to-cell fusion, and to transfer DNA directly between different species. Outlines the protocols for bacterial, fungal, and protist species that are common in research or important to human disease; identifies references to related species; and discusses theory and instrumentation to aid in designing or modifying protocols. Companion volumes will cover plant and animal cells. Claw bound.
Annotation c. Book News, Inc., Portland, OR (booknews.com)
Table of Contents
Electroporation Theory: Concepts and Mechanisms. Instrumentation. Direct Plasmid Transfer Between Bacterial Species and Electrocuring. Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation. Production of cDNA Libraries by Electroporation. Electroporation of RNA into Saccharomyces cerevisiae. Electrofusion of Yeast Protoplasts. Escherichia coli Electrotransformation. Electrotransformation in Salmonella. Electrotransformation of Pseudomonas. Electroporation of Xanthomonas. Transformation of Species with Suicide and Broad Host-Range Plasmids. Electroporation of Francisella tularensis. A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation. Genetic Transformation of Bacteroides spp. Using Electroporation. Electrotransformation of Agrobacterium. Electroporation of Helicobacter pylori. Electrotransformation of Streptococci. Transformation of Lactococcus by Electroporation. Transformation of Lactobacillus by Electroporation. Electrotransformation of Staphylococci. Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine. Introduction of Recombinant DNA into Clostridium spp. Electroporation of Mycobacteria. Electrotransformation of the Spirochete Borrelia burgdorferi. Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation. Ten-Minute Electrotransformation of Saccharomyces cerevisiae. Electroporation of Schizosaccharomyces pombe. Gene Transfer by Electroporation of Filamentous Fungi. Transformation of Candida maltosa by Electroporation. Electroporation of Physarum polycephalum. Electroporation of Dictyostelium discoideum. Gene Transfer by Electroporation of Tetrahymena. Transfection of the African and American Trypanosomes. Electroporation in Giardia lamblia. Index.
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