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Liposome Methods and Protocols (Methods in Molecular Biology)

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Liposome Methods and Protocols (Methods in Molecular Biology) Cover

 

Synopses & Reviews

Publisher Comments:

In vitro utilization of liposomes holds great potential as a powerful tool for drug targeting, gene transport across plasma and nuclear membranes, and enzyme therapy for patients with genetic disorders. In Liposome Methods and Protocols, a panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology and realizing this promise. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposomes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid. The emphasis is on clearly presenting the technical steps critical to experimental success that are too often omitted from methods published in the primary literature. State-of-the-art and highly practical, Liposome Methods and Protocols provides an indispensable collection of proven techniques and references for researchers across many fields, including glycoproteins, glycolipids, glycosyltransferases, drug transport, viral transport, antibody delivery, synthetic peptide delivery to cells, and protease delivery.

Book News Annotation:

Laboratory techniques for elucidating liposome biology are described in step-by-step detail to aid researchers working in drug targeting, gene transport across plasma and nuclear membranes, and enzyme therapy for patients with genetic disorders. Each protocol is presented by an investigator familiar with its problems and capabilities, and includes a list of materials, troubleshooting tips, and pitfalls to avoid. The protocols are of interest to researchers in glycoproteins, glycolipids, antibody delivery, and protease delivery. The editors are affiliated with the Department of Chemistry and Biochemistry at the University of Notre Dame.
Annotation c. Book News, Inc., Portland, OR (booknews.com)

Synopsis:

A panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposmes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid.

Table of Contents

Part I. Physical Properties Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids Subroto Chatterjee and Dipak K. Banerjee Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems Massimo Masserini, Paola Palestini, Marina Pitto, Vanna Chigorno, and Sandro Sonnino Part II. Liposome Fusion/Modulation Peptide-Induced Fusion of Liposomes Eve-Isabelle Pécheur and Dick Hoekstra Liposomes: Applications in Protein-Lipid Interaction Studies, Sujoy Ghosh and Robert Bell Lipids in Viral Fusion Anu Puri, Maite Paternostre, and Robert Blumenthal Part III. Application of Liposomes Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells Shimon Gatt, Tama Dinur, and Arie Dagan Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays Manju Basu and Subhash Basu Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis Roger K. Bretthauer and Dennis W. Welsh Therapeutic Uses of Antioxidant Liposomes William L. Stone, Shyamali Mukherjee, Milton Smith, and Salil K. Das Targeted Gene Delivery by Virosomes Debi P. Sarkar, Komal Ramani, and Sandeep K. Tyagi Liposomes Containing Ligands: Binding Specificity to Selectins Sriram Neelamegham and Khushi L. Matta Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes P. R. Satish and A. Surolia Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies Reiji Kannagi Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides Takao Taki and Naoto Oku Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins Francis J. Castellino and Eric H. Ellison Index

Product Details

ISBN:
9780896038455
Editor:
Basu, Subhash C.
Editor:
Basu, Manju
Editor:
Basu, Subhash C.
Editor:
Basu, Manju
Author:
Basu, Manju
Author:
Basu, Subhash C.
Publisher:
Humana Press
Location:
Totowa, N.J.
Subject:
General
Subject:
Biochemistry
Subject:
Pharmacology
Subject:
Cytology
Subject:
Liposomes
Subject:
Life Sciences - Cytology
Subject:
Pharmacy
Subject:
Health and Medicine-Pharmacology
Copyright:
Edition Description:
Book
Series:
Methods in Molecular Biology (Hardcover)
Series Volume:
199
Publication Date:
20020515
Binding:
HARDCOVER
Language:
English
Illustrations:
Yes
Pages:
264
Dimensions:
229 x 152 mm 1 gr

Related Subjects

Health and Self-Help » Health and Medicine » History of Medicine
Health and Self-Help » Health and Medicine » Medical Specialties
Health and Self-Help » Health and Medicine » Pharmacology
Science and Mathematics » Biology » Cytology and Cell Biology

Liposome Methods and Protocols (Methods in Molecular Biology) New Hardcover
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Product details 264 pages Humana Press - English 9780896038455 Reviews:
"Synopsis" by , A panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposmes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid.
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