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Other titles in the Methods in Molecular Biology series:
Oxidative Stress Biomarkers and Antioxidant Protocols (Methods in Molecular Biology)by D. Armstrong
Synopses & Reviews
Oxidative stress has been linked to a variety of medical problems, including cardiovascular disease, cancer, arthritis, diabetes, and aging. Drawing on the growing volume of research into the impact of oxidative stress on pathophysiological mechanisms, Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the perturbations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format by accomplished investigators from prestigious universities, institutes, and laboratories around the world, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltammetry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols (Humana, 2002), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays. Cutting-edge and highly practical, Oxidative Stress Biomarkers and Antioxidant Protocols provides many powerful new tools for detecting oxidative stress and for investigating the possibilities for therapeutic intervention monitoring in a variety of serious diseases.
Book News Annotation:
Intended for molecular and cellular biologists, this volume contains 33 papers outlining reproducible techniques for evaluating the perturbations in cell function resulting from increased oxidative stress. It covers methods for oxidized lipids, proteins, carbohydrates, and nucleic acids, fat and water soluble vitamins, flavonoids, and metallothionein. It also details improved statistical interpretations, correcting for random measurement error. Charts, diagrams, and other images support the text.
Annotation c. Book News, Inc., Portland, OR (booknews.com)
Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.
Table of Contents
Part I. Techniques for Free Radical Derived Biomarkers. Human Xanthine Oxidoreductase Determination by a Competitive ELISA Maria Giulia Battelli and Silvia Musiani Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC Richard W. Browne and Donald Armstrong Determination of Products of Lipid Oxidation by Infrared Spectroscopy Douglas Borchman and Santosh Sinha Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry Guey-Shuang Wu and Narsing A. Rao Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies Yoji Kato and Toshihiko Osawa Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes Ninder Panesar, Yashwant G. Deshpande, and Donald L. Kaminski Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidative Stress Jason D. Morrow and L. Jackson Roberts, II Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein Audric S. Moses and Gordon A. Francis Detection of Certain Peroxynitrite-Induced DNA Modifications Hiroshi Ohshima, László Virág, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda, and Csaba Szabó Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS José A. Castro and Gerardo D. Castro Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins Bénédicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, and Roger T. Dean Rapid Determination of Glutamate Using HPLC Technology Aqeela Afzal, Mohammed Afzal, Andrew Jones, and Donald Armstrong A Rapid Method for the Quantification of GSH and GSSG in Biological Samples Mohammed Afzal, Aqeela Afzal, Andrew Jones, and Donald Armstrong Protein Carbonyl Measurement by ELISA I. Hendrikje Buss and Christine C. Winterbourn Ne-(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in Long-Lived Tissue Proteins J. Nikki Shaw, John W. Baynes, and Suzanne R. Thorpe Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis Haw-Wen Chen and Chong-Kuei Lii Oxidation of Cellular DNA Measured with the Comet Assay Andrew R. Collins and Mária Duþs inská Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis Yoshihiro Higuchi Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA Rashid Ali and Khurshid Alam Part II. Techniques for Antioxidant Biomarkers Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices Bruce S. Kristal, Karen Vigneau-Callahan, and Wayne R. Matson Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode Roberto Bravo, Dawn M. Stickle, and Anna Brajter-Toth Measurement of a-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry Elizabeth J. Parks Analysis of Tocotrienols in Different Sample Matrixes by HPLC Kalyana Sundram and Rosnah Md. Nor Measurement of b-Carotene15,15'-Dioxygenase Activity by Reverse-Phase HPLC Alexandrine During, Akihiko Nagao, and James Cecil Smith, Jr. Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress Yorihiro Yamamoto and Satoshi Yamashita Catechol- and Pyrogallol-Type Flavonoids: Analysis of Tea Catechins in Plasma Keizo Umegaki, Mituaki Sano, and Isoa Tomita Pyruvate Dehydrogenase Complex as a Marker of Mitochondrial Metabolism: Inhibition by 4-Hydroxy-2-Nonenal, Mulchand S. Patel and Lioubov G. Korotchkina Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and In Situ Oxidase Activity Leonard A. Levin Metallothionein Determination by Isocratic HPLC with Fluorescence Derivatization Shinichi Miyairi and Akira Naganuma Quantification of Oxidized Metallothionein by a Cd-Saturation Method Dominik Klein, Uma Arora, Shin Sato, and Karl H. Summer Fractionation of Herbal Medicine for Identifying Antioxidant Activity Mohammed Afzal and Donald Armstrong Designing Safer (Soft) Drugs by Avoiding the Formation of Toxic and Oxidative Metabolites Nicholas Bodor and Peter Buchwald Statistical Correction of the Area Under the ROC Curve in the Presence of Random Measurement Error and Applications to Biomarkers of Oxidative Stress Enrique F. Schisterman Index
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