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Basic Cell Culture Protocols: Third Edition (Methods in Molecular Biology)

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Basic Cell Culture Protocols: Third Edition (Methods in Molecular Biology) Cover

 

Synopses & Reviews

Publisher Comments:

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.

Synopsis:

Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, Third Edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animals cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. This book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Table of Contents

Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. Helgason Detection of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Eradication of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting Wilhelm G. Dirks and Hans G. Drexler Cytogenetic Analysis of Cell Lines Roderick A. F. MacLeod and Hans G. Drexler Human and Mouse Hematopoietic Colony-Forming Cell Assays Cindy L. Miller and Becky Lai Isolation and Culture of Murine Macrophages John Q. Davies and Siamon Gordon Isolation and Culture of Human Macrophages John Q. Davies and Siamon Gordon Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture Tomoo Ueno, Cunlan Liu, Takeshi Nitta, and Yousuke Takahama In Vitro Generation of Lymphocytes From Embryonic Stem Cells Renée F. de Pooter, Sarah K. Cho, and Juan Carlos Zúñiga-Pflücker Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region Robert A. J. Oostendorp, Kirsty Harvey, and Elaine A. Dzierzak Culture of Human and Mouse Mesenchymal Cells Emer Clarke Isolation, Purification, and Cultivation of Murine and Human Keratinocytes Frizell L. Vaughan and Ludmila I. Bernstam Isolation and Culture of Primary Human Hepatocytes Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley, and Lysiane Richert Primary Kidney Proximal Tubule Cells Mary Taub Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity John Stingl, Joanne T. Emerman, and Connie J. Eaves Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue Sharon A. Louis and Brent A. Reynolds Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Gabi Shefer and Zipora Yablonka-Reuveni Adult Ventricular Cardiomyocytes: Isolation and Culture Klaus-Dieter Schlüter and Daniela Schreiber Isolation and Culture of Primary Endothelial Cells Bruno Larrivée and Aly Karsan Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions Susan L. Cuvelier and Kamala D. Patel Isolation and Characterization of Side Population Cells Margaret A. Goodell, Shannon McKinney-Freeman, and Fernando D. Camargo Scalable Production of Embryonic Stem Cell-Derived Cells Stephen M. Dang and Peter W. Zandstra Index

Product Details

ISBN:
9781588292841
Editor:
Helgason, Cheryl D.
Editor:
Miller, Cindy
Editor:
Helgason, Cheryl D.
Editor:
Miller, Cindy
Author:
Miller, Cindy L.
Author:
Helgason, Cheryl D.
Author:
Miller, Cindy
Publisher:
Humana Press
Subject:
Cell culture
Subject:
Tissue culture
Subject:
Life Sciences - Cytology
Subject:
Cytology
Subject:
Cell biology
Subject:
Molecular biology
Subject:
Biology-Cytology and Cell Biology
Copyright:
Edition Number:
3
Edition Description:
3rd ed. 2005
Series:
Methods in Molecular Biology
Series Volume:
290
Publication Date:
20041015
Binding:
HARDCOVER
Language:
English
Illustrations:
Y
Pages:
384
Dimensions:
229 x 152 mm 1 gr

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Basic Cell Culture Protocols: Third Edition (Methods in Molecular Biology) New Hardcover
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Product details 384 pages Humana Press - English 9781588292841 Reviews:
"Synopsis" by , Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, Third Edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animals cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. This book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
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