Synopses & Reviews
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biologya series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Book News Annotation:
Glycomics is the analysis of the structure and functions of glycans
or oligosaccharides (chains of sugars) attached to biomolecules and
includes the analysis of glycoproteins, proteoglycans, glycolipids,
peptidoglycans, and lipopolysacchrarides. Packer (Macquarie U.,
Australia) and Karlsson (National U. of Ireland) present 26 chapters
providing step-by-step descriptions of methods and protocols of
glycomics. They have organized the contributions into sections
concentrating on glycoprotein and proteoglycan analysis;
glycosylation site determination methods; the use of bioinformatic
tools for analysis of mass spectrometric data on glycans; analysis of
the specific single O-GlcNAc molecule found on nuclear and
cytoplasmic proteins; and methods for measuring glycan biomolecular
interactions using glycan arrays, mass spectrometry, nuclear magnetic
resonance, antibodies, and small molecule inhibitors. As with the
rest of the series, contributions adopt a uniform format that
includes an introduction to the topic, a list of necessary materials
and reagents, step-by-step protocols, and notes on troubleshooting
and known pitfalls.
Annotation ©2009 Book News, Inc., Portland, OR (booknews.com)
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