Summer Reading Sale

Recently Viewed clear list

Original Essays | July 24, 2014

Jessica Valenti: IMG Full Frontal Feminism Revisited

It is arguably the worst and best time to be a feminist. In the years since I first wrote Full Frontal Feminism, we've seen a huge cultural shift in... Continue »
  1. $11.90 Sale Trade Paper add to wish list


On Order

New Trade Paper
Currently out of stock.
Add to Wishlist
available for shipping or prepaid pickup only
Qty Store Section
- Local Warehouse Health and Medicine- Anatomy and Physiology

Methods in Molecular Biology #29: Chromosome Analysis Protocols


Methods in Molecular Biology #29: Chromosome Analysis Protocols Cover


Synopses & Reviews

Publisher Comments:

Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.

Table of Contents

Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and Banding. Chromosome Banding and Identification: Absorption Staining. Chromosome Banding and Identification: Fluorescence. Chromosome Banding: Stain Combinations for Specific Regions. Inhibition of Chromosome Condensation. Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity. Automated Karyotype Analysis. Bivariate Chromosome Analysis Using a Commercial Flow Cytometer. Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping. Electrophoretic Karyotype Analysis: Pulse Field Gel Electrophoresis. Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations. Immunocytochemical Techniques Applied to Meiotic Chromosome Preparations. Immortalized Cell Lines: Their Creation and Use in Gene Mapping. Oligonucleotide-Primed In Situ DNA Synthesis: An Alternative to In Situ Hybridization for Gene Mapping and the Investigation of Genome Organization. Electron Microscopic Localization of In Situ Hybrids. Protocols for Chromosome Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping. Construction of Chromosome Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines. Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints. Chromosome Dissection and Cloning. Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization. Molecular Analysis of Chromosome Aberrations: In Situ Hybridization. Alu- and L1-Primed PCR-Generated Probes for Non-Isotopic In Situ Hybridization. Chromosome Substructure Investigation: Telomeres. Index.

Product Details

Gosden, John R.
Humana Press
Human genetics
Health and Medicine-Anatomy and Physiology
Edition Description:
Softcover reprint of hardcover 1st ed. 1994
Methods in Molecular Biology
Series Volume:
Publication Date:
229 x 152 mm 724 gr

Related Subjects

Health and Self-Help » Health and Medicine » Anatomy and Physiology
Health and Self-Help » Health and Medicine » Medical Specialties
Science and Mathematics » Biology » Genetics

Methods in Molecular Biology #29: Chromosome Analysis Protocols New Trade Paper
0 stars - 0 reviews
$170.50 Backorder
Product details 520 pages Springer - English 9781617370052 Reviews:
  • back to top
Follow us on...

Powell's City of Books is an independent bookstore in Portland, Oregon, that fills a whole city block with more than a million new, used, and out of print books. Shop those shelves — plus literally millions more books, DVDs, and gifts — here at