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Peptide Arrays on Membrane Supports: Synthesis and Applications

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Peptide Arrays on Membrane Supports: Synthesis and Applications Cover

 

Synopses & Reviews

Publisher Comments:

Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.

Table of Contents

SPOT-Synthesis - Scope of Applications; Frank, Schneider-Mergener.- Chemistry of Fmoc Peptide Synthesis on Membranes; Zander, Gausepohl.- Manual Peptide Synthesis; Petersen.- Automated Synthesis of Solidphase Bound Peptides; Gausepohl, Behn.- Epitope Mapping of Antibodies with Solidphase Oligopeptides; Koch, Mahler, Blüthner. - Protein/Protein Interactions; Groves, Sinning.- Analysis of Protein/DNA Interactions; Reuter.- Affinity Purification and Competition Assays Using Solidphase Oligopeptides; Mahler, Blüthner, Koch.- Mutational Analysis and Structure Predictions; Blüthner, Koch, Mahler.- Modification of Immobilized Peptides; Bodem, Blüthner.- Immobilized Peptides to Study Protein-Protein Interactions - Potential and Pitfalls; Bräuning, Mahler, Hügle-Dörr, Blüthner, Koch, Petersen.

Product Details

ISBN:
9783540425328
Other:
Koch, Joachim
Publisher:
Springer
Author:
Koch, Joachim
Author:
Mahler, Michael
Author:
Koch, J.
Location:
Berlin, Heidelberg
Subject:
Microbiology
Subject:
Laboratory Medicine
Subject:
Peptide synthesis.
Subject:
Combinatorial Chemistry Techniques
Subject:
Life Sciences - Biochemistry
Subject:
Protein microarrays
Subject:
Oligonucleotide Array Sequence Analysis
Subject:
Chemistry-Biochemistry
Subject:
Biochemistry
Subject:
Epitop-Kartierung
Subject:
Peptidsynthese
Subject:
Proteine
Subject:
Proteininteraktionen
Subject:
binding site
Subject:
Epitope Mapping.
Subject:
protein interaction
Subject:
Biochemistry, general
Subject:
Molecular Medicine
Subject:
Pharmacology/Toxicology
Subject:
Life sciences
Subject:
Language, literature and biography
Subject:
Biomedical and Life Sciences
Subject:
Medicine
Subject:
Toxicology
Copyright:
Edition Description:
Book
Series:
Springer Lab Manuals
Series Volume:
107-27
Publication Date:
20020408
Binding:
TRADE PAPER
Language:
English
Illustrations:
Yes
Pages:
183
Dimensions:
235 x 155 mm 590 gr

Related Subjects

Health and Self-Help » Health and Medicine » Medical Specialties
History and Social Science » Law » General
Science and Mathematics » Biology » Molecular
Science and Mathematics » Chemistry » Biochemistry

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