NATO Science Series II: Mathematics, Physics and Chemistry #245: Evolving Methods for Macromolecular Crystallography: The Structural Path to the Understanding of the Mechanism of Action of Cbrn Agents

X-ray crystallography is the pre-eminent technique for visualizing the structures of macromolecules at atomic resolution. These structures are central to understanding the detailed mechanisms of biological processes, and to discovering novel therapeutics using a structure-based approach. As yet, structures are known for only a small fraction of the proteins encoded by human and pathogenic genomes. To counter the myriad modern threats of disease, there is an urgent need to determine the structures of the thousands of proteins whose structure and function remain unknown. This volume draws on the expertise of leaders in the field of macromolecular crystallography to illuminate the dramatic developments that are accelerating progress in structural biology. Their contributions span the range of techniques from crystallization through data collection, structure solution and analysis, and show how modern high-throughput methods are contributing to a deeper understanding of medical problems.

This volume draws on the expertise of leaders in the field of macromolecular crystallography to illuminate the dramatic developments that are accelerating progress in structural biology. Their contributions span the range of techniques from crystallization through data collection, structure solution and analysis. The book shows how modern high-throughput methods are contributing to a deeper understanding of medical problems.

Preface. Succeeding with Seeding: some practical Advice; T. Bergfors.- Expression, Purification and Crystallisation of membrane Proteins; Bernadette Byrne.- Macromolecular Cryo-Crystallography; E. Garinan.- Processing Diffraction Data with Mosfim; A.G. W. Leslie and H.R. Powell.- SAD Phasing: basic Concepts and High-Throughput; G.M. Sheldrick.- Likelihood-based experimental phasing in Phaser; A.J. McCoy, L. C. Storoni and R. J. Read.- Stochastic molecular Replacement; N. M. Glykos.- Likelihood-based molecular Replacement in Phaser; R. J. Read et al.- Automated Structure Determination with PHENIX ; P. D. Adam et al.- Density Modification in MAIN; D. Turk.- Ab initio Phasing starting from Low Resolution; V.Lunin et al. Structural Genomics of Mycobacterium tuberculosis - a Search for Function and new Drug Targets; T. Baker.- 3D Domain Swapping and its Relevance to conformational Diseases; M. Jaskolski.- Structural Bioinformatics: From PROTEIN Structure to Function; J.D. Watson et al.- Single-particle Imaging; D. Sayre.-