Synopses & Reviews
This is the definitive source of information on techniques for the identification and sequencing of old DNA (pieces) and their use in biological and medical research and application. Application of aDNA techniques are useful tools for investigations reaching from evolutionary studies to law enforcement approaches. What brings them together is the interest in specific methods of handling aDNA, i.e. elaborated PCR and sequencing techniques and the interpretation of the results. This books serves as an ideal guideline for it demonstrates how problem-solving strategies can be applied in various areas.
Review
From the reviews: "This is a book that does what it says on the cover. Written by one of the leaders in the aDNA field, it covers both the basic techniques and the applications of aDNA in archaeological, anthropological and zoological studies. Susanne Hummel's writing is clear, she has chosen excellent diagrams to illustrate her text, and she includes many references to help the reader find their way into the primary literature. On the whole, she is to be congratulated for producing such a comprehensive guide ... ." (Terence A. Brown and Keri A. Brown, Human Genetics, Vol. 114 (1), 2003) "This book is a valuable introduction to the field of ancient DNA analysis and of direct relevance to the forensic community. Many of the problems and challenges that the author addresses are identical to those increasingly being faced by forensic biologists ... . In this respect the great value of the book is its extensive descriptions of the methodologies and strategies ... . I strongly recommend Ancient DNA Typing as a valuable reference source to anyone required to push current DNA testing methods ... ." (Peter R Gunn, Australian Journal of Forensic Science, Vol. 35, 2003)
Review
From the reviews:
"This is a book that does what it says on the cover. Written by one of the leaders in the aDNA field, it covers both the basic techniques and the applications of aDNA in archaeological, anthropological and zoological studies. Susanne Hummel's writing is clear, she has chosen excellent diagrams to illustrate her text, and she includes many references to help the reader find their way into the primary literature. On the whole, she is to be congratulated for producing such a comprehensive guide ... ." (Terence A. Brown and Keri A. Brown, Human Genetics, Vol. 114 (1), 2003)
"This book is a valuable introduction to the field of ancient DNA analysis and of direct relevance to the forensic community. Many of the problems and challenges that the author addresses are identical to those increasingly being faced by forensic biologists ... . In this respect the great value of the book is its extensive descriptions of the methodologies and strategies ... . I strongly recommend Ancient DNA Typing as a valuable reference source to anyone required to push current DNA testing methods ... ." (Peter R Gunn, Australian Journal of Forensic Science, Vol. 35, 2003)
Synopsis
The past twenty-five years have seen huge leaps forward in the use of DNA typing in the analysis of ancient and degraded remains. Susanne Hummel describes this book as a comprehensive manual', textbook and working tool' studying the technical and methodological aspects of DNA typing. In discussing methods, strategies, protocol and applications, notably those devised and used by the Gottingen Laboratory, she provides a specialist guide for those working in the fields of anthropology, archaeology, forensic medicine, conservation biology, environmental science and food engineering.
Synopsis
Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.
Table of Contents
Introduction.- DNA markers.- aDNA extraction.- PCR.- Analysis of PCR products.- Authenticity of results.- Applications.- Protocols.- References.- Appendix.- I Basic laboratory equipment.- II PCR Troubleshooting.- Subject Index.