Synopses & Reviews
Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory “lore” that is part of the industry’s oral tradition. The Modern Biotechnology Industry: A Broad Overview, The Business of Biotechnology: The Transformation of Knowledge into Products, Pharmaceutical/Biopharmaceutical Products, Introduction to Product Quality Systems, Biotechnology and the Regulation of Food and Medical Products, Documentation, the Foundation of Quality, Quality Systems in the Production Facility, Quality Systems in the Laboratory, Introduction to a Safe Workplace, Working Safely in the Laboratory: General Considerations and Physical Hazards, Working Safely with Chemicals, Working Safely with Biological Materials, Basic Math Techniques, Proportional Relationships, Relationships and Graphing, Descriptions of Data (Descriptive Statistics),Introduction to Quality Laboratory Measurements, Tests and Assays, Introduction to Instrumental Methods and Electricity,The Measurement of Weight, The Measurement of Volume, The Measurement of Temperature, The Measurement of pH, Selected Ions and Conductivity, Measurements Involving Light A. Basic Principles and Instrumentation, Introduction to Quality Laboratory Tests and Assays, Measurements Involving Light B. Applications and Methods, Preparation of Laboratory Solutions A: Concentration Expressions and Calculations, Preparation of Laboratory Solutions B. Basic Procedures and Practical Information, Solutions: Associated Procedures and Information, Laboratory Solutions to Support the Activity of Biological Macromolecules, Culture Media for Intact Cells, Introduction to Filtration, Introduction to Centrifugation, Introduction to Bioseparations, Computers: An Overview, Data Handling with Computers, Applications of the Internet to Biotechnology. Itended for those interested in learning the basics of laboratory methods for biotechnology
Synopsis
This manual provides a systematic and very practical introduction to the bioscience laboratory. It will serve both as a primary text for undergraduate students preparing for a career in biotechnology and as a reference for laboratory professionals. It includes coverage of:
- Math in the laboratory
- Preparing solutions
- Measurements
- Safety
- Separations
- Using computers in the lab
- Quality control
- Data analysis
About the Author
LISA SEIDMAN received her Ph.D. from the University of Wisconsin and has taught for over twelve years in the Biotechnology Laboratory Technician Program at Madison Area Technical College, Madison, Wisconsin.
CYNTHIA MOORE received her doctoral degree from Temple University School of Medicine and has a career both in research and as a science educator. She is currently a faculty member in the Department of Biological Sciences at Illinois State University, Normal, Illinois.
The authors welcome comments and feedback from readers. You can e-mail Lisa Seidman at [email protected] and Cynthia Moore at [email protected].
Table of Contents
I. INTRODUCTION TO THE BIOTECHNOLOGY WORKPLACE. 1. Biotechnology and the Workplace. 2. Introduction to a Safe Workplace.
3. Biotechnology and the Regulation of Medical and Food Products.
II. PRODUCT QUALITY AND BIOTECHNOLOGY. 4. Introduction to Product Quality Systems.
5. Documentation: The Foundation of Quality.
6. Quality Systems in the Laboratory.
7. Quality Systems in the Production Facility.
III. MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW. 8. Basic Math Techniques.
9. Proportional Relationships.
10. Relationships and Graphing.
IV. DATA IN THE LABORATORY. 11. Descriptions of Data.
12. The Collection of Meaningful Data; Avoiding Errors in the Laboratory.
V. LABORATORY MEASUREMENTS. 13. Introduction to Quality Laboratory Measurements, Tests, and Assays.
14. Introduction to Instrumental Methods and Electricity.
15. The Measurement of Weight.
16. The Measurement of Volume.
17. The Measurement of Temperature.
18. The Measurement of pH, Selected Ions, and Conductivity.
19. Measurements Involving Light A: Basic Principles and Instrumentation.
20. Measurements Involving Light B: Applications and Methods.
VI. LABORATORY SOLUTIONS. 21. Preparation of Laboratory Solutions A: Concentration Expressions and Calculations.
22. Preparation of Laboratory Solutions B: Basic Procedures and Practical Information.
23. Laboratory Solutions to Support the Activity of Biological Macromolecules and Intact Cells.
24. Solutions: Associated Procedures and Information.
VII. BASIC SEPARATION METHODS. 25. Introduction to Filtration.
26. Introduction to Centrifugation.
27. Introduction to Bioseparations.
VIII. SAFETY IN THE LABORATORY. 28. Working Safely in the Laboratory: General Considerations and Physical Hazards.
29. Working Safely with Chemicals.
30. Working Safely with Biological Materials.
IX. COMPUTERS IN THE LABORATORY. 31. Computers: An Overview.
32. Data Handling with Computers.
33. Telecommunications and the Internet.