Synopses & Reviews
The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, this lab manual assumes no prior organic chemistry course has been taken.
About the Author
Professor and Chair, Consultant Environmental Microbiology
Table of Contents
Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Microscopic MeasurementsPart 2 Survey of Microorganisms5 Protozoa, Algae, and Cyanobacteria6 Microscopic Invertebrates7 Aseptic Technique8 The Bacteria9 Fungi: Yeasts and MoldsPart 3 Microscope Slide Techniques10 Negative Staining11 Smear Preparation12 Simple Staining13 Capsular Staining14 Gram Staining15 Spore Staining: Two Methods16 Acid-Fast Staining: Ziehl-Neelsen Method17 Motility DeterminationPart 4 Culture Methods18 Culture Media Preparation19 Pure Culture Techniques20 Cultivation of Anaerobes21 Bacterial Population Counts22 Slide Culture: Molds23 Slide Culture: Autotrophs24 Bacteriophage: Isolation and CulturePart 5 Environmental Influences and Control of Microbial Growth25 Temperature: Effects on Growth26 Temperature: Lethal Effects27 pH and Microbial Growth28 Osmotic Pressure and Bacterial Growth29 Ultraviolet Light: Lethal Effects30 Evaluation of Disinfectants: The Use-Dilution Method31 Evaluation of Antiseptics: The Filter Paper Disk Method32 Evaluaiton of Alcohol: Its Effectiveness as a Skin Degerming Agent33 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method34 Oligodynamic Action35 Bacterial Mutagenicity and Carcinogenesis: The Ames Test36 Effectiveness and Hand ScrubbingPart 6 Identification of Unknown Bacteria37 Preparation and Care of Stock Cultures38 Morphological Study of Unknown39 Cultural Characteristics40 Physiological Characteristics: Oxidation and Fermentation Tests41 Physiological Characteristics: Hydrolytic Reactions42 Physiological Characteristics: Miscellaneous Tests43 Use of Bergey’s Manual and Identibacter InteractusPart 7 Miniaturized Multitest Systems44 Enterobacteriaceae Identification: The API 20E System45 Enterobacteriaceae Identification: The Enterotube II System46 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System47 Staphylococcus Identification: The API Staph-Ident SystemPart 8 Microbiology of Water, Food, and Milk48 Bacteriological Examination of Water Qualtative Tests49 Bacteriological Examination of Water: The Membrane Filter Method50 Standard Plate Count of Milk51 Microbiology of Yogurt Production52 Microbiology of Alcohol Fermentation53 Bacterial Counts of FoodsPart 9 Medical Microbiology and Immunology54 A Synthetic Epidemic55 The Staphylococci: Isolation and Identification56 The Streptococci: Isolation and Identification57 Gram-Negative Intestinal Pathogens58 Urinary Tract Pathogens59 Slide Agglutination: Serological Typing60 Slide Agglutination (Latex) Test: For S. aureus Identification61 Tube Agglutination Test: The Heterophile Antibody Test62 Tube Agglutination: The Widal Test63 Phage Typing64 White Blood Cell Study: The Differential WBC Count65 Blood Grouping66 The Snyder Caries Susceptibility TestAppendix A TablesAppendix B Indicators, Stains, ReagentsAppendix C MediaAppendix D Identification ChartsAppendix E The StreptococciAppendix F Identibacter interactus