Synopses & Reviews
The book comprises ten chapters written by experts in the field on cell-cell interactions and their role in biology and medicine. Cell-cell interactions are the means by which cells are able to communicate, transfer information, develop spatial awareness and coordinate their differentiation. The ten areas have been selected for their breadth and relevance to modern research scientists where cell-cell interactions have been shown to play a critical role in biological processes. Chapters include methodologies for specialised cell types (keratinocytes, leukocytes, neurons, endothelial cells), specialised domains of membrane contact (adhesion molecules, gap junctions, tight junctions), different developmental models (Drosophila, Xenopus, mouse) and, for mammalian development, distinct stages of medical importance (oocyte-granulosa cells; preimplantation embryos; implantation). Throughout, molecular, cellular, biochemical and physiological protocols are provided in comprehensive detail to help the reader develop new skills and understanding.
Review
"Approaches and techniques are described for use in studying cell-cell interactions, focusing on biological processes where cell-cell interactions have been shown to play a critical role. Methodologies are given for working with specialized cell types, specialized domains of membrane contact, different developmental models of mammalian development, and distinct stages of medical importance. Protocols span molecular, cellular, biochemical, and physiological techniques. This second edition reflects recent developments in classical procedures. Fleming teaches in the School of Biological Sciences at the University of Southampton, UK."--SciTech Book News
Table of Contents
1. Cadherin adhesion regulation in keratinocytes,
Vania Braga2. Analysis of intercellular adhesion molecules in endothelial cells, Gianfranco Bazzoni et al
3. Gap junction mediated interactions between cells, David Becker and Colin Green
4. Functional analysis of the tight junction, Marcelino Cereijido, Liora Shoshani and Ruben Contreras
5. Leukocyte cell adhesion interactions, Carl Gahmberg et al
6. Studying cell interactions during development of the nervous system in Drosophila, David Shepherd et al
7. Tight junction protein expression in early Xenopus development and protein interaction studies, Sandra Citi et al
8. Oocyte-granulosa cell interactions, Barbara C Vanderhyden
9. Cell-cell interactions in early mammalian development, Tom Fleming et al
10. Model Systems for the investigation of implantation, John Aplin et al