Synopses & Reviews
Gene therapy is emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability of DNA in the physiological milieu limits its therapeutic potential. Nanotechnology based non viral vectors have been proposed as potential candidates to efficiently transport DNA to the target cells. Various polymeric nanoparticles have been shown to be suitable, with high cellular uptake efficiencies and reduced cytotoxicity. Dr. Surendra Nimesh gives a systematic account of the many aspects of nanotechnology-mediated gene therapy, from the preparation of nanoparticles to physicochemical characterization, and follows with applications in in vitro and in vivo models. In the concluding chapters he provides exhaustive details on polymeric systems employed for gene therapy.
About the Author
Surendra Nimesh is UGC Assistant Professor at Central University of Rajasthan, India. His research interests include nanoparticles-mediated gene, siRNA and drug delivery.
Table of Contents
Nanotechnology: An introduction Methods of nanoparticle preparation Tools and techniques for physico-chemical characterization of nanoparticles Characterization of nanoparticles: In vitro and in vivo Theory and limitations to gene therapy Targeted gene delivery mediated by nanoparticles Polymeric nanoparticles for gene delivery Poly-L-lysine nanoparticles Chitosan nanoparticles Polyethylenimine nanoparticles Atelocollagen Protamine nanoparticles Dendrimers Cyclodextrins and cyclodextrin- containing polymers Poly(D,L-lactide- co-glycolide)-based nanoparticles Metallic and inorganic nanoparticles