Synopses & Reviews
Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable.
Synopsis
Protein microarrays can overcome the sensitivity and sample limitations of functional proteomics. This volume is an exhaustive and up-to-date presentation of robust protein microarray strategies that furthers the advance of this powerful laboratory technology.
Table of Contents
Part I: Reverse Phase Protein Arrays
1. Reverse Phase Protein Microarrays for Clinical Applications
Mariaelena Pierobon, Claudio Belluco, and Lance A. Liotta, and Emanuel F. Petricoin III
2. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays
Kristi Ambroz
3. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology
Virginia Espina, Claudius Mueller, and Lance A. Liotta
4. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays
Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, and Özgür Sahin
5. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification
Jan C. Brase, Heiko Mannsperger, Holger Sültmann, and Ulrike Korf
6. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models
Satoshi Nishizuka
7. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays
Georg Martiny-Baron, Dorothea Haasen, Daniel D’Dorazio, Johannes Voshol, and Doriano Fabbro
8. Use of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues